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U6382

Sigma-Aldrich

Anti-UCP-1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Ucp1 Antibody, Ucp1 Antibody - Anti-UCP-1 antibody produced in rabbit, Anti-Uncoupling Protein 1

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About This Item

MDL number:
MFCD01092514
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

300

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~32 kDa

species reactivity

mouse, human, rat

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested sections of mouse brown adipose tissue.
western blot: 1:1,000 using mitochondrial extract of rat brown adipose tissue

UniProt accession no.

P25874

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... UCP1(7350)
mouse ... Ucp1(22227)
rat ... Ucp1(24860)

General description

Uncoupling protein (UCP1) is a 32 kDa, mitochondrial inner membrane protein. It is active as a proton channel forming dimer. It can bind purine nucleotides and is capable of being stimulated by fatty acids.

Specificity

Anti-UCP-1 specifically recognizes human, mouse and rat UCP-1. Staining of the UCP-1 band is specifically inhibited with the immunizing peptide. The epitopes recognized are compatible with routine formalin-fixation and paraffin-embedding.

Immunogen

synthetic peptide corresponding to human UCP1 sequence (amino acids 145-159) with N-terminal cysteine added, conjugated to KLH. The corresponding sequence is identical in rat and mouse UCP1.

Application

Anti-UCP-1 antibody produced in rabbit has been used in western blotting and immunohistochemistry.

Biochem/physiol Actions

Uncoupling proteins (UCPs) are transporters functioning as enzymatic uncouplers of oxidative phosphorylation. They can return protons pumped by the respiratory chain to the mitochondrial matrix. UCP-1 is exclusively expressed in brown adipose tissue (BAT) in rodents and in neonates where it is regulated by norepinephrine and thyroid hormones. UCP-1 has been shown to depend on CoQ (ubiquinone) as an obligatory co-factor.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Maria Chondronikola et al.
Diabetes, 63(12), 4089-4099 (2014-07-25)
Brown adipose tissue (BAT) has attracted scientific interest as an antidiabetic tissue owing to its ability to dissipate energy as heat. Despite a plethora of data concerning the role of BAT in glucose metabolism in rodents, the role of BAT
Towards a molecular understanding of adaptive thermogenesis
Lowell BB, et al.
Nature, 404(6778), 652-660 (2000)
The molecular features of uncoupling protein 1 support a conventional mitochondrial carrier-like mechanism
Crichton PG, et al.
Biochimie, 134(12), 35-35 (2017)
The chemical uncoupler 2, 4-dinitrophenol (DNP) protects against diet-induced obesity and improves energy homeostasis in mice at thermoneutrality
Goldgof M, et al.
The Journal of Biological Chemistry, 289(28), 19341-19350 (2014)
Severe burn injury induces thermogenically functional mitochondria in murine white adipose tissue
Porter C, et al.
Shock, 44(3), 258-258 (2015)
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