Skip to Content
Merck
CN
All Photos(1)

Documents

I3909

Sigma-Aldrich

Ionomycin calcium salt

Ready Made Solution, from Streptomyces conglobatus, 1 mM in DMSO

Synonym(s):

Calcium Ionophore Ionomycin

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C41H70CaO9
Molecular Weight:
747.07
EC Number:
200-664-3
MDL number:
MFCD00083634
UNSPSC Code:
51101500
PubChem Substance ID:
329815372
NACRES:
NA.85

biological source

Streptomyces conglobatus

Quality Level

300

Assay

≥98% (HPLC)

shelf life

2 yr

storage condition

(Tightly closed. )

concentration

1 mM in DMSO

color

colorless

mp

18.4 °C

solubility

DMSO: 1 mM

antibiotic activity spectrum

Gram-positive bacteria

Mode of action

cell membrane | interferes

shipped in

wet ice

storage temp.

−20°C

SMILES string

C[C@@H]1CCC(=O)O[Ca]OC(=CC(=O)[C@@H](C)C[C@@H](C)C1)[C@H](C)C[C@H](C)C\C=C\[C@@H](C)[C@@H](O)[C@@H](C)[C@@H](O)C[C@@H]2CC[C@](C)(O2)[C@H]3CC[C@](C)(O3)[C@@H](C)O

InChI

1S/C41H72O9.Ca/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42;/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47);/q;+2/p-2/b13-11+,34-24-;/t25-,26-,27+,28-,29-,30+,31+,32-,33+,36+,37-,39-,40+,41+;/m1./s1

InChI key

WKRWUYKLUMMAKG-WYGBAUISSA-L

Related Categories

General description

Ionomycin is a calcium ionophore commonly derived from produced by the bacterium Streptomyces conglobatus, which means that it helps to transport calcium ions across cell membranes. It is a powerful tool for studying calcium signaling and its role in various biological processes, including cell activation, secretion, proliferation, and apoptosis.

Ionomycin is commonly used in cell biology experiments to induce a rapid and controlled increase in intracellular calcium levels. This allows to study the downstream effects of calcium signaling pathways, such as the activation of enzymes, the release of hormones, and the changes in gene expression.Ionomycin is also used to investigate the role of calcium in specific cellular processes, such as cell death, immune cell activation, and muscle contraction. For example, ionomycin can be used to induce apoptosis in cancer cells, study the activation of T cells, and investigate the role of calcium in muscle contraction.

Application

Ionomycin finds application in:
  • Study of calcium signaling and its role in various biological processes
  • Investigation of intracellular calcium levels, calcium-dependent enzymes, and cellular responses triggered by calcium influx
  • Induction of a rapid and controlled increase in intracellular calcium levels in cell biology experiments
  • Elucidation of specific cellular mechanisms and signaling pathways in combination with other substances, such as fluorescent calcium indicators or protein kinase activators
Ionomycin has been used:
  • to induce central demyelination
  • inhibit adrenal bovine TREK-1 channels
  • to regulate cell division of mature human B cells

Biochem/physiol Actions

Ionomycin is an ionophoric antibiotic that binds Pb2+ and Ca2+, and serves as an effective mobile carrier of both cations.

Mode of Action: Facilitates the transfer of calcium ions (Ca2+) across biological membranes

Antimicrobial Spectrum: Active against Gram-Positive bacteria
Ionomycin is an ionophoric antibiotic which binds Pb2+ and Ca2+, and serves as an effective mobile carrier of both cations. Ionomycin is more effective than A23187 as a mobile ion carrier for Ca2+. It is non-fluorescent. It is used to study Ca2+ transport across biological membranes and induces apoptotic degeneration of embryonic cortical neurons.

Features and Benefits

  • Ready available solution reduce the need for preparation time
  • Efficient choice for studying calcium signaling and its role in various biological processes
  • Allows for rapid and controlled manipulation of intracellular calcium levels
  • Versatile and widely used in cell biology research

Other Notes

For additional information on our range of Biochemicals, please complete this form.

WGK

WGK 1

Flash Point(F)

closed cup

Flash Point(C)

closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. Is Product No. I3909 (Ionomycin calcium salt solution) hygroscopic?

    Yes, this product is hygroscopic.  It is packaged under an inert gas.  It is recommended to store I3909 under an inert gas and at -20°C protected from light.

  7. What is the concentration of the Ionomycin calcium salt solution, Product No. I3909?

    It is a 1mM solution or ~ 0.75mg/mL in DMSO.

  8. Has Ionomycin calcium salt solution, Product No. I3909, been tested for use in Cell Culture applications?

    Sigma Aldrich has not tested this material for use in cell culture.  However, several references for use of ionomycin in cell culture applications can be found in the product information sheet (under Documents, above).

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

K M Kim et al.
Journal of immunology (Baltimore, Md. : 1950), 148(6), 1797-1803 (1992-03-15)
The growth of a human B lymphoma cell line B104, an experimental model for mature B cells, was inhibited by ionomycin but not 12-O-tetradecanoylphorbol-13-acetate (TPA). Ionomycin inhibited B104 cells from entering into the M phase of the cell cycle without
K J Smith et al.
Brain : a journal of neurology, 117 ( Pt 6), 1351-1356 (1994-12-01)
The effects of injecting the calcium-selective ionophore, ionomycin, into myelinated tracts in the dorsal columns of adult rat spinal cords were examined electron microscopically. In vivo, ionomycin induced a primary vesicular demyelination, together with a variable degree of axonal degeneration
Thorsten Schäfer et al.
Allergy, 65(10), 1242-1255 (2010-03-27)
Mast cells are secretory immune cells whose degranulation can provoke acute allergic reactions. It is presently unclear, however, whether an individual mast cell can repeatedly degranulate or turns dysfunctional after a single antigen stimulus. This work thus aims to better
John J Enyeart et al.
American journal of physiology. Cell physiology, 301(3), C619-C629 (2011-05-27)
Bovine adrenocortical cells express bTREK-1 K(+) (bovine KCNK2) channels that are inhibited by ANG II through a Gq-coupled receptor by separate Ca(2+) and ATP hydrolysis-dependent signaling pathways. Whole cell and single patch clamp recording from adrenal zona fasciculata (AZF) cells
Shakti Singh et al.
PLoS pathogens, 15(8), e1007460-e1007460 (2019-08-06)
Candida auris is an emerging, multi-drug resistant, health care-associated fungal pathogen. Its predominant prevalence in hospitals and nursing homes indicates its ability to adhere to and colonize the skin, or persist in an environment outside the host-a trait unique from
View All Related Papers

Articles

A Highly Sensitive IFN-γ ELISpot Assay to Quantify Cellular Immune Responses to Previous Viral Infection

This study presents an example of a typical ELISpot assay for quantifying the number of pre-existing, antigen-reactive T cells from peripheral blood mononuclear cell (PBMC) samples obtained from donors previously infected with cytomegalovirus (CMV).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service