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Key Documents

567531-U

Supelco

Discovery® HS F5 (3 µm) HPLC Columns

L × I.D. 10 cm × 4 mm, HPLC Column

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1 EA
¥9,028.06

¥9,028.06

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1 EA
¥9,028.06

About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

¥9,028.06

Please contact Customer Service for Availability

Product Name

Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 10 cm × 4 mm

material

stainless steel column

Quality Level

100

Agency

suitable for USP L43

product line

Discovery®

feature

endcapped

manufacturer/tradename

Discovery®

packaging

1 ea of

extent of labeling

12% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

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This Item
567532U567530U567501U
Discovery ® HS F5 HPLC 色谱柱 3 μm particle size, L × I.D. 10 cm × 4 mm

567531U

Discovery ® HS F5 HPLC 色谱柱

Discovery ® HS F5 HPLC 色谱柱 3 μm particle size, L × I.D. 15 cm × 4 mm

567532U

Discovery ® HS F5 HPLC 色谱柱

Discovery ® HS F5 HPLC 色谱柱 3 μm particle size, L × I.D. 5 cm × 4 mm

567530U

Discovery ® HS F5 HPLC 色谱柱

Discovery ® HS F5 HPLC 色谱柱 3 μm particle size, L × I.D. 3.3 cm × 2.1 mm

567501U

Discovery ® HS F5 HPLC 色谱柱

matrix

silica gel, high purity, spherical particle platform, fully porous particle

matrix

silica gel, high purity, spherical particle platform, fully porous particle

matrix

silica gel, high purity, spherical particle platform, fully porous particle

matrix

silica gel, high purity, spherical particle platform, fully porous particle

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

matrix active group

PFP (pentafluorophenyl) phase

matrix active group

PFP (pentafluorophenyl) phase

matrix active group

PFP (pentafluorophenyl) phase

matrix active group

PFP (pentafluorophenyl) phase

particle size

3 μm

particle size

3 μm

particle size

3 μm

particle size

3 μm

pore size

120 Å

pore size

120 Å

pore size

120 Å

pore size

120 Å

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

General description

Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.

Features and Benefits

  • Unique selectivity
  • Similar retention to C18 (sometimes requires stronger mobile phase)
  • Excellent peak shape
  • Stable, low-bleed LC-MS separations
  • Scalable separations from 3 to 10μm particle sizes

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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Description
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Certificates of Analysis (COA)

Lot/Batch Number
280937-06
280937-05
280937-04
280937-03
280937-02

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Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 48(2), 254-263 (2007-12-14)
In this study a pentafluorophenylpropyl (PFPP) stationary phase was applied to the fast and reliable qualitative and quantitative analysis of ephedrine alkaloids in Ephedra plant material and derivatives. A Discovery HS F5 column (150mmx4.6mm i.d., 5microm) was used, with an
Ilia Brondz et al.
Journal of pharmaceutical and biomedical analysis, 43(3), 937-944 (2006-11-03)
The drug primaquine diphosphate is used for causative treatment of malaria. Using HPLC-MS and GC-MS, this research group was previously able to show that the main contaminant of primaquine is the positional isomer quinocide [I. Brondz, D. Mantzilas, U. Klein
Federica Pellati et al.
Journal of chromatography. A, 1165(1-2), 58-66 (2007-08-07)
In this study, the chromatographic performance of a pentafluorophenylpropyl (PFPP) stationary phase was evaluated for the rapid separation of phenethylamine alkaloids (i.e. (+/-)-octopamine, (+/-)-synephrine, tyramine, N-methyltyramine and hordenine) in Citrus aurantium plant material (fruits and peel), various Citrus species, extracts
Hiroyuki Kataoka et al.
Analytical and bioanalytical chemistry, 405(1), 331-340 (2012-10-16)
We have developed a simple and sensitive method for the simultaneous determination of testosterone (TES), cortisol (CRT), and dehydroepiandrosterone (DHEA) in saliva by automated online in-tube solid-phase microextraction (SPME) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a Discovery HS
Koji Baba et al.
Journal of chromatography. A, 1354, 109-116 (2014-06-21)
Arsenic species, including arsenous acid, arsenic acid, methylarsonic acid, and dimethylarsinic acid, were determined using HPLC-ICPMS. The species were separated with a Discovery HS F5 column and a simple, volatile, and isocratic mobile phase of 0.1% (v/v) formic acid and
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