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AB3381

Sigma-Aldrich

Anti-Myosin Light Chain Antibody, phospho-specific (Ser19)

Chemicon®, from rabbit

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UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

NCBI accession no.

NM_002475.3

UniProt accession no.

P14649

shipped in

dry ice

target post-translational modification

phosphorylation (pSer19)

Gene Information

human ... MYBPHL(343263)

Specificity

Myosin, human, smooth and non-muscle regulatory light chain, phosphoSer19. By Western blot the antibody recognizes the ~20 kDa phosphorylated protein. The immunogen sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain. The antibody recognizes monophosphorylated (pSer19) and diphosporylated (pThr18-pSer19) forms of the protein. Reactivity to non-phosphorylated human myosin, smooth and non-muscle regulartory light chain is minimal (less than 1%) by ELISA.

Immunogen

Epitope: phospho-specific (Ser19)
Synthetic phosphopeptide from human myosin, smooth and non-muscle regulatory light chain.

Application

Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-Myosin Light Chain Antibody, phospho-specific (Ser19) is validated for use in ELISA, WB for the detection of Myosin Light Chain.
Western blot: 1:1,000-1:5,000 using ECL.
ELISA: 1:120,000-1:135,000
Immunohistochemistry: 2.5 μg/mL

Physical form

Affinity purified immunoglobulin. Liquid in 0.02M Potassium phosphate, pH 7.2 with 0.15 M Sodium chloride and 0.01% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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