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T8787

Sigma-Aldrich

Triton™ X-100

for molecular biology

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Synonym(s):
t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
9036-19-5
Beilstein:
2315025
MDL number:
MFCD00128254
UNSPSC Code:
12161900
PubChem Substance ID:
329826885
NACRES:
NA.21

biological source

synthetic (organic)

Quality Level

200

grade

for molecular biology

description

non-ionic

form

liquid

mol wt

micellar avg mol wt 80,000
average mol wt 625

aggregation number

100-155

technique(s)

protein purification: suitable
protein quantification: suitable
western blot: suitable

impurities

<1.00% water (Karl Fischer)

pH

9.7

CMC

0.2-0.9 mM (20-25°C)

mp

6 °C

transition temp

cloud point 65 °C
pour point ~7 °C

solubility

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

density

1.06 g/mL at 25 °C (lit.)

cation traces

Fe: <5 ppm
K: <0.05%
Na: <0.1%
heavy metals: <5 ppm

HLB

13.5

application(s)

hematology
histology

foreign activity

DNase and RNase, none detected

storage temp.

room temp

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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General description

Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton™ X-100 has been used:
  • In immunohistochemistry for staining the Flat-mount retinas
  • Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay
  • To permeabilise cells during Immunofluorescent microscopic studies
  • As a positive control in LDH assay to determine the cell membrane integrity
  • For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure
  • For the preparation of outer membrane protein exctract
  • As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells
  • In the treatment of tissue sections for Immunofluorescence labeling

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Features and Benefits

  • Ideal for sensitive molecular biology research applications
  • Tested for DNase and RNase
  • Non-ionic nondenaturing detergent
  • Superior wetting agent and emulsifier
  • Highly versatile surfactant

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
For additional information on our range of Biochemicals, please complete this form.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

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Description
Pricing

Signal Word

Danger

Hazard Statements

H302,H315,H318,H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Membrane dielectric changes indicate induced apoptosis in HL-60 cells more sensitively than surface phosphatidylserine expression or DNA fragmentation.
Wang X, et al.
Biochimica et Biophysica Acta - Biomembranes, 1564(2), 412-420 (2002)
M A Ferguson et al.
Journal of applied physiology (Bethesda, Md. : 1985), 85(3), 1169-1174 (1998-09-08)
The purpose of this study was to determine the threshold of exercise energy expenditure necessary to change blood lipid and lipoprotein concentrations and lipoprotein lipase activity (LPLA) in healthy, trained men. On different days, 11 men (age, 26.7 +/- 6.1
Jean-Sébastien Joyal et al.
Blood, 117(22), 6024-6035 (2011-03-01)
The failure of blood vessels to revascularize ischemic neural tissue represents a significant challenge for vascular biology. Examples include proliferative retinopathies (PRs) such as retinopathy of prematurity and proliferative diabetic retinopathy, which are the leading causes of blindness in children
Eric Soupene et al.
Experimental biology and medicine (Maywood, N.J.), 233(5), 507-521 (2008-04-01)
Acyl-CoA synthetase enzymes are essential for de novo lipid synthesis, fatty acid catabolism, and remodeling of membranes. Activation of fatty acids requires a two-step reaction catalyzed by these enzymes. In the first step, an acyl-AMP intermediate is formed from ATP.
Mehul B Shah et al.
Methods in molecular biology (Clifton, N.J.), 398, 21-28 (2008-01-25)
Raft and caveolar microdomains have been proposed to participate in numerous cellular functions including signal transduction, cholesterol trafficking, and vesicular sorting. Traditional methods of isolation of rafts from cultured cells and tissue samples have exploited the biochemical properties of these
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