70856
RNase A Solution
Convenient solution for selective degradation of RNA
Sign Into View Organizational & Contract Pricing
All Photos(1)
UNSPSC Code:
12352202
NACRES:
NA.54
Recommended Products
form
solution
manufacturer/tradename
Novagen®
storage condition
OK to freeze
concentration
10 mg/mL
technique(s)
DNA purification: suitable
shipped in
ambient
storage temp.
10-30°C
General description
RNase A Solution is a convenient alternative to powdered RNase A. It is a highly purified preparation of bovine pancreatic ribonuclease A suitable for use in selective removal of RNA. It has been pretreated to remove DNase I and is suitable to remove RNA contamination during plasmid and genomic DNA purification procedures. Supplied at a concentration of 10 mg/ml in 10 mM Tris-HCl, 1 mM EDTA, 50% glycerol, pH 7.5.
Application
RNase A Solution has been used:
- in cell cycle profiling of embryonic mouse neuroectodermal cells (NE-4C)
- in the digestion of RNA for extraction of DNA from human osteosarcoma cell lines for chromatin immunoprecipitation (ChIP) assays
- to treat control adult and embryonic thymi cells during staining
- to remove RNA from affibody–mRNA fusion clones before clone screening
Biochem/physiol Actions
RNaseA is an endoribonuclease that catalyzes the degradation of phosphodiester linkages with a pyrimidine base at the 3′ -position on single-stranded RNA via, transphosphorylation and hydrolysis mechanism.
Warning
Toxicity: Standard Handling (A)
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Myc controls a distinct transcriptional program in fetal thymic epithelial cells that determines thymus growth
Jennifer E C, et al.
Nature Communications (2019)
Neurotrophic properties and the de novo peptide sequencing of edible bird's nest extracts
Yew M Y, et al.
Food Bioscience, 32, 100466-100466 (2019)
Jiajun Zhang et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 26(11), 2681-2692 (2020-01-16)
Malignancy of cancer cells depends on the active transcription of tumor-associated genes. Recently, unique clusters of transcriptional enhancers, termed super-enhancers, have been reported to drive the expression of genes that define cell identity. In this study, we characterized specific super-enhancer-associated
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service