产品名称
核糖核酸酶 A 溶液, Convenient solution for selective degradation of RNA
form
solution
manufacturer/tradename
Novagen®
storage condition
OK to freeze
concentration
10 mg/mL
technique(s)
DNA purification: suitable
shipped in
ambient
storage temp.
10-30°C
Application
RNA酶A溶液已用于：
- 小鼠胚胎神经外胚层细胞(NE-4C)的细胞周期分析
- 人骨肉瘤细胞系DNA提取实验中的RNA消化，以便进行染色质免疫沉淀(ChIP)分析
- 在染色时处理成人和胚胎胸腺细胞的对照细胞
- 从亲和体中去除RNA–克隆筛选前的mRNA融合克隆
Biochem/physiol Actions
RNaseA是一种核糖核酸内酶，通过转磷酸化和水解机制催化单链RNA上3′位嘧啶基磷酸二酯键的降解。
Disclaimer
毒性：标准处理（A）
General description
RNase A溶液是粉末状RNase A的方便替代品。它是牛胰核糖核酸酶A的高度纯化制剂，适用于选择性去除RNA。它已经经过预处理去除DNase I，适用于在质粒和基因组DNA纯化过程中去除RNA污染。在10 mM Tris-HCl，1 mM EDTA，50%甘油，pH 7.5中以10 mg/ml的浓度提供。
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
10 - Combustible liquids
wgk
WGK 2
Neurotrophic properties and the de novo peptide sequencing of edible bird's nest extracts
Yew M Y, et al.
Food Bioscience, 32, 100466-100466 (2019)
Myc controls a distinct transcriptional program in fetal thymic epithelial cells that determines thymus growth
Jennifer E C, et al.
Nature Communications (2019)
Jiajun Zhang et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 26(11), 2681-2692 (2020-01-16)
Malignancy of cancer cells depends on the active transcription of tumor-associated genes. Recently, unique clusters of transcriptional enhancers, termed super-enhancers, have been reported to drive the expression of genes that define cell identity. In this study, we characterized specific super-enhancer-associated
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