KitAlysis High-Throughput Palladium Precatalyst Cross-Coupling Reaction Screening Kit

Materials Included in your KITALYSIS-24PD-2PK High-Throughput Screening Kit

Contents in each of the 2 individually sealed Mylar (foil bags):

• 24 pre-weighed catalysts in glass vials loaded with stir bars, topped with cap mat.

• 4 empty reaction vials with lids (to make stock solutions)

The screening sets come pre-loaded with 1 µmol of catalyst in each vial according to the following design.

Mouse over image to select.

Internal Standard:

• Biphenyl (30 mg). To be added to the reaction during the work-up. Recipe for the work-up with the internal standard can be found within the hyperlinked screen types in the "Kit Design" section below.

KitAlysis™ 24-well Reaction Block Replacement Films-2EA

• Pack of 2 enables a new film to be used with each kit ensuring a tight, cross-contaminate-free seal every time.

Stir Bars-3 individually packed

• Fit perfectly into supplied vials to ensure proper stirring of substrate mixtures prior to dosing.
  

Additional Recommended Materials (Sold Separately)

• Z688711: 96-well plate for automated HPLC analysis
• C1111: 96-well plate cap mat for automated HPLC analysis
• Z683809: 10-100 µL pipette
• Z640220: 2-200 µL pipette tip refill
• Z683825: 100-1000 µL pipette
• Z640247: 50-1000 µL pipette tip refill
• Z192570: 2 inch needles for ease in ampule solvent extraction
• Z230723: 1 mL needle for accurate solvent volume extraction from ampule

Kit Design

The 24 palladium precatalyst cross-coupling kit was designed to provide the best possible chance of finding good reaction conditions for a wide range of cross-coupling reactions and is meant to widen the chemical space offered in more tailored kits (e.g Suzuki, Buchwald-Hartwig) or to allow you to customize a reaction of your choice.

A step-by-step user guide and an excel sheet for stock solution recipes can be downloaded within the step-by-step user guide hyperlinked above.

Use of the Provided Tools

Multiple tools have been created to ensure your success with kit set up. Start with the more detailed guide to ensure you are comfortable with all of the steps before using the quick guides on the excel worksheet. Remember that while the technique is new, it is still organic chemistry and so the steps will seem easy once you try just one kit. It is just a new way of approaching something you are already very good at.

Detailed Set-Up Guide:

As this kit is designed to allow the user to customize based upon their needs, there is no specific user guide provided but some best practices, order of operations, how-to’s for the Labware, and work-up recipes with procedures. Everything you need to set up a kit with confidence every time.

Additionally, consulting the existing detailed user guides may be helpful if you are looking to extend an existing kit (for example Suzuki-Miyaura & Buchwald Hartwig) or to get inspiration. Consulting the Cross-Coupling Guide will also provide you with some additional places to start. There is also a very useful video to aid in set-up. All of these resources either combined or in isolation can provide general trouble-shooting.

Excel Sheet:

The downloadable excel file is general, allowing the user to design their experiment to their specifications. For the most general reaction types, guidelines are provided in the detailed reaction guides for the kits already available in the KitAlysis™ portfolio and in the Cross-Coupling guide. The excel sheet provides:

• Calculations for substrate recipes depending upon the molecular weight of your substrates
• Quick directions for the more avid user
• Print button to allow you to take recipe to the lab
• Contains all info such that they can be can be saved as a pdf and appended to ELN for experimental information