LC/MS Analysis of Sorbitol and Mannitol on SeQuant® ZIC-HILIC
Conditions | |
|---|---|
| column | SeQuant® ZIC-cHILIC, PEEK, 250 x 4.6 mm, 3 μm, 100Ã… (1.50662.0001) |
| column temp. | 45 °C (oven), 40 °C (detector cell) |
| mobile phase | [A] acetonitrile; [B] 0.1% ortho-phosphoric acid in Milli-Q® water; (90:10, A:B) |
| flow rate | 1.5 mL/min |
| pressure | 1682 psi (116 bar) |
| sample | 5.0 mg/mL (5000 ppm) each of sorbitol and mannitol in diluent. (Diluent: Acetonitrile: 0.1% ortho-phosphoric acid in Milli-Q® water 75:25 (v/v)) |
| injection | 20 μL |
| detector | Refractive Index (RI) (cell: 10 μL) |
Description | |
|---|---|
| Featured Industry | Pharmaceutical (small molecule) |
| suitability | application for HPLC |
Materials
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