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column	Ascentis^® Express OH5, 10 cm x 3.0 mm I.D., 2.7 µm particles (53769-U)
column temp.	25 °C
mobile phase	[A] acetonitrile; [B] water with 0.1% formic acid, pH 3.5 with ammonium formate; (75:25, A:B)
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Analysis Note	L-methionine sulfoximine and L-buthionine sulfoximine are used in are used to prevent additional enzyme activity in Chinese hampster ovary (CHO) cell lines with supplemental Glutamine Synthase (GS). It is of interest to be able to monitor both purity of such molecules in order to control addition to cell media as well as for the assay of parent molecules during use. The analytes are highly polar and thus should be amenable to HILIC-LC-MS analysis. This first installment investigates the ability of Ascentis Express HILIC and Ascentis^® Express OH5 stationary phases for the separation of the methionine analog and related compounds as well as methionine and buthionine separations.
Categories	Analytical Chromatography, Amino Acids
Featured Industry	Chemicals and Industrial Polymers
Legal Information	Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability	application for HPLC

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