Western Blot Analysis of Immunoprecipitation (IP-Western)
IP-Western analysis remains a popular technique for identifying protein-protein interactions and identifying unknown proteins in a multi-protein complex. The steps include cell lysis, formation of the antibody-antigen (immune) complex, precipitation of the immune complexes, and analysis by Western blotting.
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Figure 1.High background, leading to nonspecific bands on Western blot.
Figure 2.In a cross-linking IP western, the heavy and light chains of the primary capture antibody are excluded from the sample.
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