Cyramza induces apoptosis of HCC4006 cell by affecting the level of Bcl-w.

Lung cancer seriously threats to patient's life and health. Cyramza is a therapeutic drug for inhibition of vessel formation and growth in clinical practice. The aim of this study was to investigate the effect of cyramza on growth and apoptosis of non-small lung cancer HCC4006 and explore the related mechanisms. Cell viability and apoptosis were examined by MTT assay and flow cytometry, respectively. Western blot was employed to examine the effect of cyramza on the apoptotic protein Bcl-w. After that Bcl-w knockdown and overexpression were respectively fulfilled by Bcl-w siRNA and plasmid transfection, effects of cyramza on cell apoptosis were determined by Western-blot. Cyramza inhibited the cell growth and induced the cell apoptosis in HCC4006 cells, which was mediated by downregulation of Bcl-w level. Bcl-w knockdown and overexpression could increase and decrease the effect of cyramza on cell apoptosis, respectively. Cyramza induced the apoptosis of non-small lung cancer cell line HCC4006 via the downregulation of Bcl-w.