- Photochemistry and mechanism of designed pyrenyl probe towards promoted cleavage of proteins.
Photochemistry and mechanism of designed pyrenyl probe towards promoted cleavage of proteins.
Journal of photochemistry and photobiology. B, Biology (2017-05-30)
Sudarat Yenjai, Mayuso Kuno, Siritron Samosorn, Teerayuth Liwporncharoenvong, Apinya Buranaprapuk
PMID28554074
摘要
A new photochemical reagent, succinic acid-1(1-pyrene)methylamide (PMA-SUC), was developed to recognize the specific binding sites on model proteins, egg-white lysozyme and avidin. The interaction of the photochemical reagent with the proteins was studied by UV-Vis, fluorescence spectroscopic methods and docking description. PMA-SUC was found to bind to lysozyme and avidin with binding constants (K
材料