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Merck
CN
  • A complementary strategy for enhancement of nanoparticle intracellular uptake.

A complementary strategy for enhancement of nanoparticle intracellular uptake.

Pharmaceutical research (2014-02-22)
Yingjia Li, Ge Wen, Dongxiao Wang, Xia Zhang, Yaoyong Lu, Jianguo Wang, Lijuan Zhong, Hongbing Cai, Xingmei Zhang, Ying Wang
摘要

The complementary strategy by combining targeting ligand-mediated selectivity and CPP-mediated transmembrane function could be exploit synergies for enhancing cellular uptake of nanoparticles with negative charge. A heparin-based nanoparticles with negative charge was fabricated by complementary strategy, which was expected to attain efficient uptake and simultaneously exert great anticancer activity. We synthesized heparin-based nanoparticles with targeting ligand folate and CPP ligand Tat to deliver paclitaxel (H-F-Tat-P NPs). The NPs were characterized by (1)H NMR, DLS and TEM, respectively. The effect of dual ligands on system behavior in aqueous solution was investigated. Moreover, its cellular internalization and anticancer activity were detected by flow cytometry, confocal microscopy and MTT. Folate played a key role in the formation of heparin-based NPs dependent on the balance of amphiphilic Tat and hydrophobic folate. Although H-F-Tat-P NPs primarily entered FR specific and non-specific cells by similar routes, there were no comparability due to cell-type specific variation. Unlike non-specific cells, the complementary ligands could help negative-charged NPs to enhance cellular uptake facilitating its endosome escape in specific cells thereby exhibiting great anticancer activity. The complementary strategy for negative-charged NPs was presented a promising delivery system for diverse anticancer agents enable simultaneously targeting and drug delivery.

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Sigma-Aldrich
尸胺, 95%
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Sigma-Aldrich
尸胺, purum, ≥97.0% (GC)
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Supelco
尸胺, analytical standard
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50 discs
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