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Merck
CN
  • Highly sensitive impedimetric immunosensor based on single-walled carbon nanohorns as labels and bienzyme biocatalyzed precipitation as enhancer for cancer biomarker detection.

Highly sensitive impedimetric immunosensor based on single-walled carbon nanohorns as labels and bienzyme biocatalyzed precipitation as enhancer for cancer biomarker detection.

Biosensors & bioelectronics (2014-01-15)
Fan Yang, Jing Han, Ying Zhuo, Zhehan Yang, Yaqin Chai, Ruo Yuan
摘要

A novel sandwich-type electrochemical immunosensor based on functionalized nanomaterial labels and bienzyme (horseradish peroxidase and glucose oxidase) biocatalyzed precipitation was developed for the detection of α-fetoprotein (AFP). The enzymes linked to functionalized nanomaterials as biocatalysts could accelerate the oxidation of 4-chloro-1-naphthol (4-CN) by H2O2 to yield the insoluble product on the electrode surface; the mass loading of the precipitates on the device led to a significant enhanced signal. Cyclic voltammetry and electrochemical impedance spectroscopy techniques were used to monitor the enhanced precipitation of 4-CN that accumulated on the electrode surface and subsequent decrement in the electrode surface area by monitoring the reduction process of the Fe(CN)6(4-/3-) redox couple. Under optimal conditions, the proposed immunosensor showed a high sensitivity and a wide linear range from 0.001 to 60 ng mL(-1) with a low detection limit of 0.33 pg mL(-1). Moreover, the immunosensor exhibited good selectivity, acceptable stability and reproducibility. The amplification strategy showed good promise for clinical screening of tumor biomarkers.

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Sigma-Aldrich
Carbon nanohorns, as-grown
Sigma-Aldrich
Carbon nanohorns, oxidized, avg. no. of layers, 1