- A multiwell assay for inhibitors of phosphatidylinositol-3-kinase and the identification of natural product inhibitors.
A multiwell assay for inhibitors of phosphatidylinositol-3-kinase and the identification of natural product inhibitors.
Anticancer research (1994-11-01)
T Frew, G Powis, M Berggren, R T Abraham, C L Ashendel, L H Zalkow, C Hudson, S Qazia, E Gruszecka-Kowalik, R Merriman
PMID7872663
摘要
A convenient and reliable multisample assay for the screening of inhibitors of the growth factor signalling enzyme phosphatidylinositol-3-kinase (PtdIns-3-K) has been developed. Four natural product inhibitors of Ptdlns-3-K have been identified with IC50 values for hypericin 0.18 microM, emodin 3.3 microM, asperuloside 2.0 microM and uttronin A 1.1 microM.
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