- Structural plasticity broadens the specificity of an engineered protease.
Structural plasticity broadens the specificity of an engineered protease.
Nature (1989-05-18)
R Bone, J L Silen, D A Agard
PMID2716847
摘要
The substrate specificity of alpha-lytic protease has been changed dramatically, with a concomitant increase in activity, by replacing an active-site Met with Ala. The substrate specificity of both this mutant and another similar mutant are extraordinarily broad. X-ray crystallographic analysis shows that structural plasticity, a combination of alternate side-chain conformations and binding-site flexibility, allows both large and small substrates to be well accommodated.