[HPLC determination of diperodon enantiomers in blood serum by using teicoplanin chiral stationary phase].

[HPLC determination of diperodon enantiomers in blood serum by using teicoplanin chiral stationary phase].

Ceska a Slovenska farmacie : casopis Ceske farmaceuticke spolecnosti a Slovenske farmaceuticke spolecnosti (2004-10-28)

K Hrobonová, J Lehotay, J Cizmárik

摘要

The chiral stationary phase on the base of teicoplanin and the polar-organic mobile phase methanol/acetonitrile/acetic acid/triethylamine 45/55/0.3/0.2 was used for the separation of diperodon enantiomers. The developed method was suitable to determine the enantiomers in blood serum up to 0.5 microg/ml. The degradation of diperodon enantiomers was studied in serum by an in vitro method and the experimental rate constants were determined.