[Synthesis of new fragments of VP1 protein fragments from foot and mouth disease virus type A22. Synthesis of fragments 134-139, 134-145, 140-145, 150-155, and 150-159].

Fragments 134-145 and 150-159 of the antigenic-region of the VP1 protein of the A22 foot-and-mouth disease virus were synthesized by classic methods of peptide chemistry with isobutyl chloroformate as a coupling reagent. After purification by HPLG and amino acid analysis, the free peptides H-Gly-Lys-Tyr-Ser-Ala-Gly-Gly-Leu-Gly-Arg-Arg-Gly-OH and H-Leu-Ala-Ala-Arg-Val-Ala-Lys-Gln-Leu-Pro-OH were conjugated with BSA by means of N,N-dicyclohexylcarbodiimide. The conjugates were used, with complete Freund adjuvant, for immunization on guinea pigs. The antibodies formed had a virus neutralization activity.