- NIR-to-NIR two-photon excited CaF2:Tm3+,Yb3+ nanoparticles: multifunctional nanoprobes for highly penetrating fluorescence bio-imaging.
NIR-to-NIR two-photon excited CaF2:Tm3+,Yb3+ nanoparticles: multifunctional nanoprobes for highly penetrating fluorescence bio-imaging.
ACS nano (2011-10-01)
Ning-Ning Dong, Marco Pedroni, Fabio Piccinelli, Giamaica Conti, Andrea Sbarbati, Juan Enrique Ramírez-Hernández, Laura Martínez Maestro, Maria Carmen Iglesias-de la Cruz, Francisco Sanz-Rodriguez, Angeles Juarranz, Feng Chen, Fiorenzo Vetrone, John A Capobianco, José García Solé, Marco Bettinelli, Daniel Jaque, Adolfo Speghini
PMID21957870
摘要
In this study, we report on the remarkable two-photon excited fluorescence efficiency in the "biological window" of CaF(2):Tm(3+),Yb(3+) nanoparticles. On the basis of the strong Tm(3+) ion emission (at around 800 nm), tissue penetration depths as large as 2 mm have been demonstrated, which are more than 4 times those achievable based on the visible emissions in comparable CaF(2):Er(3+),Yb(3+) nanoparticles. The outstanding penetration depth, together with the fluorescence thermal sensitivity demonstrated here, makes CaF(2):Tm(3+),Yb(3+) nanoparticles ideal candidates as multifunctional nanoprobes for high contrast and highly penetrating in vivo fluorescence imaging applications.