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Merck
CN
  • Antibody-dependent enhancement (ADE) of SARS-CoV-2 pseudoviral infection requires FcγRIIB and virus-antibody complex with bivalent interaction.

Antibody-dependent enhancement (ADE) of SARS-CoV-2 pseudoviral infection requires FcγRIIB and virus-antibody complex with bivalent interaction.

Communications biology (2022-03-26)
Shuang Wang, Junchao Wang, Xiaojuan Yu, Wen Jiang, Shuo Chen, Rongjuan Wang, Mingzhu Wang, Shasha Jiao, Yingying Yang, Wenbo Wang, Huilin Chen, Ben Chen, Chunying Gu, Chuang Liu, An Wang, Min Wang, Gang Li, Cuicui Guo, Datao Liu, Jinchao Zhang, Min Zhang, Lan Wang, Xun Gui
摘要

Understanding the underlying molecular mechanisms behind ADE of SARS-CoV-2 is critical for development of safe and effective therapies. Here, we report that two neutralizing mAbs, MW01 and MW05, could enhance the infection of SARS-CoV-2 pseudovirus on FcγRIIB-expressing B cells. X-ray crystal structure determination and S trimer-binding modeling showed that MW01 and MW05 could bind to RBDs in S trimer with both "up" and "down" states. While, the neutralizing mAb MW07, which has no ADE activity only binds to RBD in S trimer with "up" state. Monovalent MW01 and MW05 completely diminished the ADE activity compared with their bivalent counterparts. Moreover, both macropinocytosis and endocytosis are confirmed involving in ADE of SARS-CoV-2 pseudoviral infection. Blocking endosome transportation and lysosome acidification could inhibit the ADE activity mediated by MW05. Together, our results identified a novel ADE mechanism of SARS-CoV-2 pseudovirus in vitro, FcγRIIB-mediated uptake of SARS-CoV-2/mAb complex with bivalent interaction.

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Sigma-Aldrich
佛波醇12-十四酸酯13-乙酸酯, ≥99% (TLC), film or powder
Sigma-Aldrich
Mix-n-染色 CF 488A 抗体标记试剂盒(50-100μg)