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Merck
CN

Selection of recombinant antibodies by eukaryotic ribosome display.

Methods in molecular biology (Clifton, N.J.) (2008-07-02)
Mingyue He, Michael J Taussig
摘要

Ribosome display is a powerful method for selection of single-chain antibodies in vitro. It operates through the formation of libraries of antibody-ribosome-mRNA complexes that are selected on immobilized antigen, followed by recovery of the genetic information from the mRNA by RT-PCR. Both prokaryotic and eukaryotic versions are used. We describe our eukaryotic system, in which rabbit reticulocyte extracts are used for cell free transcription/translation and cDNA is recovered by in situ RT-PCR performed on the selected complexes.

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Sigma-Aldrich
2'-脱氧胞苷 5'-三磷酸 二钠盐, 100 mM (pH 7)