- [Degradation of collagen by the cariogenic bacteria, Streptococcus mutans].
[Degradation of collagen by the cariogenic bacteria, Streptococcus mutans].
Comptes rendus des seances de l'Academie des sciences. Serie D, Sciences naturelles (1980-01-07)
S Despres, H Métivier, R Weill
PMID6248261
摘要
The behaviour of cariogenic Bacteria (Streptococcus mutans) is studied with regard to collagen, which represents 90% of the dentine organic matrix. Collagenase activity of cariogenic Bacteria is measured with radioactive precursors and gel electrophoresis and compared to reference bacterial collagenase (Clostridium histolyticum). Labelled collagen substrate has been prepared with two different methods: extraction by 0,5 M acetic acid from young Rat skin, previously labelled with L-proline 14C, or reduction by Na B3H4. Both collagen sutstrates have been incubated for 2 h in Terleckyj medium in which the Streptococcus mutans have been inoculated. The experiments show a proteolytic activity of Streptococcus Mutans on the collagen substrate.
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胶原酶 来源于溶组织梭菌, non-sterile; 0.2 μm filtered, Type IA-S, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid