Human Intestinal Enteroids Model MHC-II in the Gut Epithelium.

Human Intestinal Enteroids Model MHC-II in the Gut Epithelium.

Frontiers in immunology (2019-09-05)

Jonathan E Wosen, Alexandra Ilstad-Minnihan, Julia Y Co, Wei Jiang, Dhriti Mukhopadhyay, Nielsen Q Fernandez-Becker, Calvin J Kuo, Manuel R Amieva, Elizabeth D Mellins

PMID31481960

摘要

The role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, "mini-guts" derived from small intestinal crypt stem cells, and show that the intracellular MHC-II peptide-pathway is intact and functional in IECs. Our approach enables human enteroids to be used for novel in vitro studies into IEC MHC-II regulation and function during health and disease.