Effect of silicone antifoam on shear sensitivity of hybridoma cells in sparged cultures.

Effect of silicone antifoam on shear sensitivity of hybridoma cells in sparged cultures.

Biotechnology progress (1993-09-01)

L A van der Pol, D Bonarius, G van de Wouw, J Tramper

摘要

Addition of high concentrations of silicone antifoam (AF) to a suspension of hybridoma cells in a bubble column reduces the death rate when using medium without a protective component. In contrast, high AF concentrations can cause detrimental effects in a sparged medium with a protective component, such as is applied for large-scale animal cell cultures.