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安全信息

C2874

Sigma-Aldrich

CryoStor® 细胞冻存培养基

CS10

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别名:
cell freezing medium
UNSPSC代码:
12352207
NACRES:
NA.75

质量水平

100

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable
cryopreservation: suitable

运输

ambient

储存温度

2-8°C

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相关类别

一般描述

CryoStor CS2、CS5 和 CS10 系列保存溶液代表了下一代冷冻保存培养基。CryoStor介质设计用于在超低温环境（-80至-196°C）中进行细胞的制备和保存，并在冷冻、储存和解冻过程中为细胞和组织提供安全的保护环境。这些培养基可调节细胞对低温储存工艺的生化反应，从而增强细胞活力和功能，同时无需血清、蛋白质或高水平细胞毒性药。

CryoStor CS2、CS5 和 CS10 是一系列细胞特异性、优化的保存培养基，独特的配方解决了冷冻保存过程中细胞的分子生物学方面；从而直接降低冷冻保存诱导的迟发性细胞死亡级别，提高解冻后细胞活力和功能。

这些培养基推荐用于干细胞、肝细胞、组织样本和其他极其敏感的细胞类型的保存。

应用

CryoStor®细胞低温储存培养基已用于：
  • T细胞保存
  • 人类诱导多能干细胞分化心肌细胞(hiPSC-CMs)保存
  • hiPSC分化心脏祖细胞(hiPSC-CPs)保存
  • 用作由牛白蛋白组成的低温储存液的成分

CryoStor 是一系列细胞特异性的、优化的冻存培养基，具有独特的配方，可在冻存过程中解决细胞的分子生物学问题，从而直接降低冻存诱导的迟发性细胞死亡，并提高解冻后细胞的活力和功能。

生化/生理作用

CryoStor® CS10是一种独特配方并含有10%二甲基亚砜（DMSO）的冷冻保存介质。推荐用于干细胞、组织样本及其他几位敏感的细胞类型的保存。

Cryostor® 冷冻保存视频操作流程

其他说明

配方含有10% DMSO。

法律信息

CryoStor is a registered trademark of BioLife Solutions, Inc.

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WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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Susie Barbeau et al.
Stem cell research, 49, 102106-102106 (2020-12-30)
Congenital myasthenic syndromes (CMS) are a class of inherited disorders affecting the neuromuscular junction, a synapse whose activity is essential for movement. CMS with acetylcholinesterase (AChE) deficiency are caused by mutations in COLQ, a collagen that anchors AChE in the
Physical events occurring during the cryopreservation of immortalized human T cells
Meneghel J, et al.
PLoS ONE, 14(5), e0217304-e0217304 (2019)
The impact of varying cooling and thawing rates on the quality of cryopreserved human peripheral blood T cells
Baboo J, et al.
Scientific reports, 9(1), 3417-3417 (2019)
In vivo maturation of human induced pluripotent stem cell-derived cardiomyocytes in neonatal and adult rat hearts
Kadota S, et al.
Stem Cell Reports, 8(2), 278-289 (2017)
Huyen Thi Lam Nguyen et al.
STAR protocols, 1(2) (2020-10-13)
Tumor organoids are promising tools for cancer biology investigations and preclinical drug screenings because they are often representative of the histology and drug responses of patients. Here, we introduce a facile protocol to overcome technical limitations by generating patient-derived tumor
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