C6295
Cell Freezing Medium-DMSO Serum free 1x
sterile-filtered, suitable for cell culture
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Synonym(s):
cryopreservation medium
UNSPSC Code:
12352207
NACRES:
NA.75
Recommended Products
sterility
sterile-filtered
Quality Level
form
liquid
technique(s)
cell culture | mammalian: suitable
cryopreservation: suitable
impurities
endotoxin, tested
shipped in
dry ice
storage temp.
−20°C
General description
Cell freezing medium is a combination of cryoprotectant DMSO with fetal bovine serum. However serum free medium replaces serum with methylcellulose and poloxamer 188 and other additives like ascorbic acid, α-tocopherol and proline.
Application
Cell Freezing Medium-DMSO Serum free 1x has been used in the freezing of human neural progenitor cells (hNPCs), olfactory ensheathing cells (OECs) and retinal pigment epithelial (RPE) cells.
Other Notes
Complete ready-to-use medium containing Minimum Essential Medium, dimethyl sulfoxide (8.7%) and methyl cellulose. Prepared according to the formulation of Waymouth.
related product
Product No.
Description
Pricing
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
动植物来源培养基
Certificates of Analysis (COA)
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Acute and delayed transplantation of olfactory ensheathing cells promote partial recovery after complete transection of the spinal cord
Lopez-Vales R, et al.
Neurobiology of Disease, 21(1), 57-68 (2006)
A novel serum-free method for culturing human prenatal retinal pigment epithelial cells
Gamm DM, et al.
Investigative Ophthalmology & Visual Science, 49(2), 788-799 (2008)
Glial cell line-derived neurotrophic factor-secreting human neural progenitors show long-term survival, maturation into astrocytes, and no tumor formation following transplantation into the spinal cord of immunocompromised rats.
Gowing G, et al.
Neuroreport, 25(6), 367-367 (2014)
Chen-Yang Meng et al.
Oncology reports, 43(4), 1169-1186 (2020-04-24)
Osteosarcoma (OS) is the most common primary malignant tumor of the bone affecting children and adolescents. Chemotherapy is now considered as a standard component of OS treatment, not only for children, but also for adults. However, chemoresistance continues to pose
Xiangwen Zhan et al.
Biochemical and biophysical research communications, 501(3), 779-785 (2018-05-16)
The proto-oncogene PIM1 encodes Ser/Thr kinase and regulates cell growth, differentiation and apoptosis. However, more and more studies including ours have found that PIM1 can induce senescence in normal human diploid fibroblasts and behave as a tumor suppressor. But the
Articles
Overview of good cell banking practices for cell line cryopreservation purposes.
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