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Merck
CN

C2874

CryoStor® cell cryopreservation media

CS10

Synonym(s):

cell freezing medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Key Documents

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Quality Level

100

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable
cryopreservation: suitable

shipped in

ambient

storage temp.

2-8°C

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General description

The CryoStor CS2, CS5, and CS10 family of preservation solutions represents the next generation of cryopreservation media. Designed to prepare and preserve cells in ultra low temperature environments (–80 to –196 °C), CryoStor media provide a safe, protective environment for cells and tissues during thefreezing, storage, and thawing process. Through modulating the cellular biochemical response to the cryopreservation process, these media provide enhanced cell viability and functionality, while eliminating the need to include serum, proteins, or high levels of cytotoxic agents.

CryoStor CS2, CS5, and CS10 are a series of cell specific, optimized preservation media, uniquely formulated to address the molecular biological aspects of cells during the cryopreservation process; thereby, directly reducing the level of Cryopreservation-Induced Delayed-Onset Cell Death and improving post-thaw cell viability and function.

These media are recommended for the preservation of stem cells, hepatocytes, tissue samples, and other extremely sensitive cell types.

Application

CryoStor, a series of cell-specific, optimized preservation media, is uniquely formulated to address the molecularbiological aspects of cells during the cryopreservation process thereby directly reducing the level of Cryopreservation-Induced Delayed-Onset Cell Death and improving post-thaw cell viability and function.
CryoStor® cell cryopreservation media has been used in:
  • the preservation of T cells
  • the preservation of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)
  • hiPSC-derived cardiac progenitors (hiPSC-CPs)
  • as a component of cryoprotective solution composed of bovine albumin

Other Notes

Formulation contains 10% DMSO.

Legal Information

CryoStor is a registered trademark of BioLife Solutions, Inc.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
MKCX4356
MKCX4355
MKCX4354
MKCX4353
MKCX4352

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In vivo maturation of human induced pluripotent stem cell-derived cardiomyocytes in neonatal and adult rat hearts
Kadota S, et al.
Stem Cell Reports, 8(2), 278-289 (2017)
Huyen Thi Lam Nguyen et al.
STAR protocols, 1(2) (2020-10-13)
Tumor organoids are promising tools for cancer biology investigations and preclinical drug screenings because they are often representative of the histology and drug responses of patients. Here, we introduce a facile protocol to overcome technical limitations by generating patient-derived tumor
Susie Barbeau et al.
Stem cell research, 49, 102106-102106 (2020-12-30)
Congenital myasthenic syndromes (CMS) are a class of inherited disorders affecting the neuromuscular junction, a synapse whose activity is essential for movement. CMS with acetylcholinesterase (AChE) deficiency are caused by mutations in COLQ, a collagen that anchors AChE in the
Physical events occurring during the cryopreservation of immortalized human T cells
Meneghel J, et al.
PLoS ONE, 14(5), e0217304-e0217304 (2019)
The impact of varying cooling and thawing rates on the quality of cryopreserved human peripheral blood T cells
Baboo J, et al.
Scientific reports, 9(1), 3417-3417 (2019)
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Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

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良好的细胞库操作实践

用于细胞系冷冻保存的良好细胞库操作实践概述。

Protocols

CryoStor® Cryopreservation Protocol

Cryopreservation affects post-thaw recovery, viability, and functionality. Stress during freezing and suboptimal media lead to cell death.

Induced Pluripotent Stem Cell Culture Protocols

Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.

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Product Information Sheet - C2874

Instructions

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