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Key Documents

Safety Information

C6039

Sigma-Aldrich

Cell Freezing Medium-Glycerol 1×

sterile-filtered, suitable for cell culture

Synonym(s):

Cell Cryopreservation Solution, Freezing Medium for Cells

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

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sterility

sterile-filtered

Quality Level

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
NaHCO3: no
HEPES: no

shipped in

dry ice

storage temp.

−20°C

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This Item
C6295C2639C6164
sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

components

L-glutamine: no
NaHCO3: no
HEPES: no

components

-

components

-

components

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

shipped in

dry ice

shipped in

dry ice

shipped in

ambient

shipped in

dry ice

General description

Cell Freezing Medium-Glycerol 1× is designed to protect and preserve cells during frozen storage. It is recommended for the cryopreservation of cells.

Application

Cell Freezing Medium-Glycerol 1× has been used for cryopreservation of honey bee semen,[1] dispersed blastomeres, and whole blastoderms.[2]

Other Notes

Complete ready-to-use medium. Proprietary formulation containing Minimum Essential Medium, glycerol, calf serum and fetal bovine serum.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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Cryopreservation of rainbow trout blastoderms
BaBIak I, et al.
Cybium, 32, 139-141 (2008)
Usage of green coconut water and different tissue culture media for in vitro honey bee semen storage (Apis mellifera; Hymenoptera: Apoidea)
Almeida R, et al.
Interciencia, 27, 317-321 (2002)

Protocols

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

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