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Merck
CN
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文件

A6559

Sigma-Aldrich

腺苷 5′-三磷酸 二钠盐 溶液

Crystalline ATP, HPLC purified, aqueous solution for RNA transcription

别名:

ATP

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About This Item

CAS号:
987-65-5
MDL编号:
MFCD00080339
UNSPSC代码:
41106305
eCl@ss:
32160414
PubChem化学物质编号:
24891106
NACRES:
NA.52

质量水平

300

形式

liquid

浓度

100 mM in H2O (adjusted to pH 7 with Trizma®
base)

异质活性

DNase, RNase, none detected

运输

dry ice

储存温度

−20°C

SMILES字符串

[Na+].[Na+].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O

InChI

1S/C10H16N5O13P3.2Na/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20;;/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20);;/q;2*+1/p-2/t4-,6-,7-,10-;;/m1../s1

InChI key

TTWYZDPBDWHJOR-IDIVVRGQSA-L

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相关类别

一般描述

腺苷-5′-三磷酸二钠盐溶液适用于分子生物学应用，如激酶反应或 DNA 依赖性 RNA 聚合酶转录。

应用

5′-腺苷三磷酸二钠盐溶液已用于：
  • 作为标准品，检测血管纹边缘细胞的ATP胞外释放
  • 作为电极内液组分，制备记录电极研究神经突伸长
  • 体外VPS34（磷脂酰肌醇 3-激酶（PtdIns3K）复合物的催化亚基）活性测定
  • 用于ATP 研究的裂解和重新密封缓冲液的成分
  • 分光光度法测量异硫氰酸荧光素 （FITC）葡聚糖负载的 A549（人腺癌肺泡基底上皮细胞）和 HeLa 细胞中的 H+ 转运
  • 诱导脂多糖处理的成纤维样滑膜细胞（FLS） 中的细胞焦亡

生化/生理作用

5′-腺苷三磷酸(ATP)是所有生物体中的重要“能量通货”(energy currency)，具有较高的磷酸转移潜力。ATP参与膜转运、肌肉收缩和合成以及生物分子降解等多种生物过程。它可作为细胞内外的信号分子，在细胞迁移、器官发育、神经传递和胰岛素分泌等细胞过程中发挥作用。

法律信息

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. Can Product A6559, Adenosine 5´-triphosphate disodium salt solution, be used in molecular biology applications?

    This is a Molecular Biology grade ATP. It is certified free of DNase and RNase. This product is recommended for any typical molecular biology application.

  7. Can this preparation of Product A6559, Adenosine 5´-triphosphate disodium salt solution, be used as an ATP standard?

    This preparation of ATP is not recommended for use as an ATP standard. The recommended ATP products for use as a standard in bioluminescence experiments is Product Code FL-AAS (preweighed vials of 1 mg each = 2 micromole).

  8. What is meant by 25UMO in the size description of Product A6559, Adenosine 5´-triphosphate disodium salt solution?  What is the product dissolved in?

    25UMO means 25 micromoles.  The 25 UMO size is equivalent to 250 μl of a 100 mM solution. This product is a 100 mM solution in water; the solution pH has been adjusted with Trizma base but contains no additional buffer or salts.

  9. Can Product A6559, Adenosine 5´-triphosphate disodium salt solution, be used for in vitro transcription?

    The purity of this product should be sufficient for use in T7 RNA polymerase-catalyzed in vitro transcription assay, but we do not test the product for use in that application in our quality control process.

  10. How long can aqueous ATP solutions like Product No. A6559 be stored?

    Aqueous solutions of ATP are stable for months when frozen in solution at -15 °C and for approximately one week at 0 °C.  As with most products, we guarantee the quality of this material for 1 year when stored unopened at the recommended storage temperature (-20°C in this case).

  11. What is the binding affinity of ATP (Product No. A6559) for Mg++ and other metal ions?

    ATP has intrinsic metal binding affinity. The binding constant for various metals are (given as per mole): Mg++, 9554; Na+, 13; Ca++, 3722; K+, 8; Sr++, 1381; Li+, 25.  The ability of ATP to chelate magnesium results in effective Mg++ ion concentrations lower than the total Mg ion concentration.  For example, in a reaction with 1.5mM Mg++ and 0.2 mM ATP, the effective Mg ion concentration is ~1.3mM.

  12. What is the millimolar extinction coefficient for ATP, Product No. A6559?

    The millimolar extinction coefficient (EmM) is 15.4, measured at 259 nm for a sample in 100 mM phosphate buffer, pH 7.0.

  13. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Cooper GM
The Cell: A Molecular Approach (2000)
James Franklin Tucker et al.
PLoS genetics, 12(2), e1005873-e1005873 (2016-02-20)
Epigenetic gene silencing plays a critical role in regulating gene expression and contributes to organismal development and cell fate acquisition in eukaryotes. In fission yeast, Schizosaccharomyces pombe, heterochromatin-associated gene silencing is known to be mediated by RNA processing pathways including
Yahui Huang et al.
Archiv der Pharmazie (2018-06-29)
Inhibition of spleen tyrosine kinase (Syk) is a promising strategy for the treatment of various allergic and autoimmune disorders such as asthma, rheumatoid arthritis, and allergic rhinitis. Previously, a Syk inhibitor with novel indazole scaffold was discovered by structure-based virtual
Hiromi Imamura et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(37), 15651-15656 (2009-09-02)
Adenosine 5'-triphosphate (ATP) is the major energy currency of cells and is involved in many cellular processes. However, there is no method for real-time monitoring of ATP levels inside individual living cells. To visualize ATP levels, we generated a series
Identification of small molecule inhibitors of phosphatidylinositol 3-kinase and autophagy.
Farkas T
The Journal of Biological Chemistry, 286(45), 38904-38912 (2011)
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