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G3776

Guanosine 5′-triphosphate sodium salt solution

Name: Guanosine 5′-triphosphate sodium salt solution
Brand: SIGMA

HPLC purified, aqueous solution for RNA polymerase transcription

Synonym(s):

GTP

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About This Item

CAS Number:

36051-31-7

NACRES:

NA.52

PubChem Substance ID:

24895138

UNSPSC Code:

41106305

MDL number:

MFCD00077781

Assay:

≥95% (HPLC)

Biological source:

Porcine brain, bacterial (Corynebacterium), yeast

Form:

liquid

Storage temp.:

−20°C

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Properties

Quality Segment

300

biological source

Porcine brain, bacterial (Corynebacterium), yeast

assay

≥95% (HPLC)

form

liquid

feature

PCR Additives

concentration

100 mM in H₂O (adjusted to pH 7)

technique(s)

PCR: suitable using : suitable, WGA

color

colorless

foreign activity

DNase, RNase and Nickase, none detected

shipped in

dry ice

storage temp.

−20°C

SMILES string

[Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]3O)C(=O)N1

InChI

1S/C10H16N5O14P3.3Na/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21;;;/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18);;;/q;3*+1/p-3/t3-,5-,6-,9-;;;/m1.../s1

InChI key

KZRMTEVIDYXWQW-CYCLDIHTSA-K

Gene Information

human ... HRAS(3265)

Description

Application

Guanosine 5′-triphosphate sodium salt solution has been used:

for reconstituting transducin during purification of transducin.
as a compnent of the 3.3X loading buffer to assemble 32P-miR-31—3xFLAG-AGO2 complexes
as a component of the nucleotide substrate mix to evaluate the hydrolytic activity of MutT homolog 1 (MTH1) on methylated nucleoside triphosphates
in DNA-dependent RNA polymerase transcription

Biochem/physiol Actions

Guanosine 5′-triphosphate (GTP) forms nearly 25% of the total nucleotide triphosphate pool and is a nucleotide anion. It participates in several biological functions such as cell signaling by activation of GTP-binding proteins, RNA and protein synthesis, and production of cyclic guanosine monophosphate (cGMP), a secondary messenger. GTP also participates in the production of stringent alarmone.

flash_point_f

No data available

flash_point_c

No data available

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Faceshields, Gloves, type N95 (US)

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