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B6755

Sigma-Aldrich

BIS-TRIS丙烷

≥99.0% (titration)

别名:

1,3-双[三(羟甲基)甲氨基]丙烷, 2,2′-(丙烷-1,3-二基双(氮烷二基))双(2-(羟甲基)丙烷-1,3-二醇)

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10 MG
CN¥1,177.86
50 MG
CN¥4,132.48
100 MG
CN¥7,415.09

CN¥1,177.86

国内现货，预计发货时间April 27, 2025详情

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10 MG
CN¥1,177.86
50 MG
CN¥4,132.48
100 MG
CN¥7,415.09

About This Item

线性分子式:
CH2[CH2NHC(CH2OH)3]2
CAS号:
64431-96-5
分子量:
282.33
Beilstein:
1786109
EC 号:
264-899-3
MDL编号:
MFCD00004689
UNSPSC代码:
12161700
PubChem化学物质编号:
24891934
NACRES:
NA.25

CN¥1,177.86

国内现货，预计发货时间April 27, 2025详情

获取大包装报价

质量水平

400

方案

≥99.0% (titration)

表单

powder

储存条件

dry at room temperature

技术

HPLC: suitable

颜色

white

有效pH范围

6.3-9.5

pKa (25 °C)

(1) 6.8, (2) 9.0

mp

164-165 °C (lit.)

溶解性

water: 30 % (w/w), clear, colorless

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此商品
Z640190Z640220Z640271
Eppendorf® epT.I.P.S. box volume range 50-1000 μL

Z640247

Eppendorf® epT.I.P.S. box

Eppendorf® epT.I.P.S. box volume range 0.1-10 μL

Z640190

Eppendorf® epT.I.P.S. box

Eppendorf® epT.I.P.S. box volume range 2-200 μL

Z640220

Eppendorf® epT.I.P.S. box

Eppendorf® epT.I.P.S. box volume range 100-5000 μL

Z640271

Eppendorf® epT.I.P.S. box

feature

barrier: no

feature

barrier: no

feature

barrier: no

feature

barrier: no

manufacturer/tradename

Eppendorf® 0030 073.100, Eppendorf® 022491351

manufacturer/tradename

Eppendorf® 0030 073.002, Eppendorf® 022491300

manufacturer/tradename

Eppendorf® 0030 073.061, Eppendorf® 022491334

manufacturer/tradename

Eppendorf® 0030 073.169, Eppendorf® 022491385

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

material

blue rack

material

dark gray rack

material

yellow rack

material

violet rack

packaging

pack of 96 (includes 1 reusable box)

packaging

pack of 96 (includes 1 reusable box)

packaging

pack of 96 (includes 1 reusable box)

packaging

pack of 24 (includes 1 reusable box)

L

71 mm

L

34 mm

L

53 mm

L

120 mm

一般描述

Bis-Tris丙烷缓冲液，也称BTP，是一种两性离子生化缓冲液，用于各种实验室应用（特别是分子生物学、生物化学和电泳）中pH的调节。其广泛的缓冲范围为pH 6.3至9.5，这一点归因于其接近的pKa值6.8 (pKa1)和 9.0 (pKa2)。这一广泛的缓冲范围令其能够在各种实验中保持稳定的pH条件。

此外，Bis-Tris丙烷是一种水溶性缓冲物质，在提高限制酶的稳定性和活性中起关键作用。具体而言，其缓冲能力对于于需要pH水平低至 6-7的实验有利。Tris 缓冲液在pH 低于7.5时缓冲能力不足，在温度变化时pKa波动很大，与其相比Bis-Tris丙烷是一种更好的选择。此外，它可与盐酸缓冲液一起用于稳定法尼基二磷酸，特别是从酿酒酵母中分离时。特别是，它可作为配体并与镧系稀土元素（III）形成双核羟基复合物。

应用

BIS-TRIS丙烷可用于以下研究：
  • 作为缓冲液用于研究具有不同亲核性质的阴离子对氧合肌红蛋白(MbO2)自动氧化的影响。[1]
  • 来自硫化叶菌膜的葡萄糖结合蛋白的纯化。[2]
  • 作为GeO2矿化过程中的缓冲液。[3]
  • 环境相关研究中的缓冲液成分[4]
  • 在工程酶的纯化和制备过程中用于维持最优pH条件的缓冲液成分。[5]

特点和优势

  • 适用于生物学和生化研究
  • 经测试确认重金属污染水平较低，确保适用于各种应用
  • 有效缓冲范围为pH 6.3-9.5 (25 °C)，pKa为 6.8 和9.0 (25 °C)
  • 易溶于水

其他说明

如需了解生化试剂系列的更多信息，请填写此表。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    0000435443
    0000435446
    0000435446
    0000435443
    0000427159

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    S V Albers et al.
    Journal of bacteriology, 181(14), 4285-4291 (1999-07-10)
    The archaeon Sulfolobus solfataricus grows optimally at 80 degrees C and pH 2.5 to 3.5 on carbon sources such as yeast extracts, tryptone, and various sugars. Cells rapidly accumulate glucose. This transport activity involves a membrane-bound glucose-binding protein that interacts
    Engineering the Organophosphorus Acid Anhydrolase Enzyme for Increased Catalytic Efficiency and Broadened Stereospecificity on Russian VX
    Daczkowski CM et al.
    Biochemistry, 54, 6423-6433 (2015)
    Y Satoh et al.
    The Journal of biological chemistry, 256(20), 10272-10275 (1981-10-25)
    In the presence of a salt in an excess amount, the oxidation of native MbO2 was enhanced considerably above the normal autoxidation in buffr alone with the formation of the corresponding MetMb . anion complex. This anion-induced oxidation of MbO2
    Estefania Boix et al.
    Dalton transactions (Cambridge, England : 2003), 43(44), 16902-16910 (2014-10-11)
    We demonstrate the use of silk based proteins to control the particle/crystallite size during GeO2 formation, using a bio-mimetic approach at circumneutral pH and ambient temperature. Multicrystalline GeO2 was prepared from germanium tetraethoxide (TEOG) in the presence of different silk-based
    Elisabeth Dohmann Chang et al.
    Environmental science & technology, 55(10), 6848-6856 (2021-03-17)
    Water pH is predicted to affect the uptake of ionizable pharmaceuticals in fish. The current study used an in vitro primary fish gill cell culture system to assess the effect of pH values in the range of 4.5-8.75 on the
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