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EHIFIP-RO

Roche

Expand™ 高保真PLUS PCR 系统

sufficient for ≤500 reactions (03300226001), sufficient for ≤2,500 reactions (03300234001), suitable for PCR, High Fidelity PCR, hotstart: no, dNTPs included: no

别名:

高保真pcr plus系统，扩展型

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500 UNITS
CN¥5,624.32

CN¥5,624.32

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500 UNITS
CN¥5,624.32

About This Item

EC 号:
2.7.7.7 (BRENDA, IUBMB)
UNSPSC代码:
41106300
NACRES:
NA.21

CN¥5,624.32

请联系客服了解存货情况

用途

sufficient for ≤2,500 reactions (03300234001)
 sufficient for 2500 reactions
 sufficient for ≤500 reactions (03300226001)
 sufficient for 500 reactions

质量水平

100

特点

High Fidelity PCR
dNTPs included: no
hotstart: no

包装

pkg of 2,500 U (03300234001 [10x250 U])
pkg of 500 U (03300226001 [2x250 U])

制造商/商品名称

Roche

参数

72 °C optimum reaction temp.

技术

PCR: suitable

输入

purified DNA

储存温度

−20°C

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此商品
EHIFIPNROEHIFIROEHIFIDNTPRO
Expand™ High FidelityPLUS PCR System sufficient for ≤500 reactions (03300226001), sufficient for ≤2,500 reactions (03300234001), suitable for PCR, High Fidelity PCR, hotstart: no, dNTPs included: no

EHIFIPRO

Expand™ High FidelityPLUS PCR System

Expand™ High FidelityPLUS PCR System, dNTPack sufficient for ≤125 reactions (04743725001), sufficient for ≤500 reactions (04743733001), suitable for PCR, dNTPs included

EHIFIPNRO

Expand™ High FidelityPLUS PCR System, dNTPack

Expand™ High Fidelity PCR System sufficient for ≤40 reactions (11732641001), sufficient for ≤200 reactions (11732650001), sufficient for ≤1,000 reactions (11759078001)

EHIFIRO

Expand™ High Fidelity PCR System

Expand™ High Fidelity PCR System, dNTPack suitable for PCR, High Fidelity PCR, hotstart: no, dNTPs included

EHIFIDNTPRO

Expand™ High Fidelity PCR System, dNTPack

usage

sufficient for ≤2,500 reactions (03300234001)

usage

sufficient for ≤125 reactions (04743725001), sufficient for ≤500 reactions (04743733001)

usage

sufficient for ≤1,000 reactions (11759078001), sufficient for ≤200 reactions (11732650001), sufficient for ≤40 reactions (11732641001)

usage

sufficient for ≤1,000 reactions (04738276001), sufficient for ≤200 reactions (04738268001), sufficient for ≤40 reactions (04738250001)

feature

High Fidelity PCR

feature

High Fidelity PCR, dNTPs included, hotstart: no

feature

-

feature

High Fidelity PCR, dNTPs included, hotstart: no

packaging

pkg of 2,500 U (03300234001 [10x250 U])

packaging

pkg of 125 U (04743725001), pkg of 500 U (04743733001 [2x250 U])

packaging

pkg of 2,500 U (11759078001 [10x250 U]), pkg of 100 U (11732641001), pkg of 500 U (11732650001 [2x250 U])

packaging

pkg of 2,500 U (04738276001 [10x250 U]), pkg of 100 U (04738250001), pkg of 500 U (04738268001 [2x250 U])

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

parameter

72 °C optimum reaction temp.

parameter

72 °C optimum reaction temp.

parameter

-

parameter

-

一般描述

Expand High FidelityPLUS PCR系统是Taq DNA聚合酶和一种缺乏聚合酶活性的热稳定性校正蛋白的混合物。
Expand High FidelityPLUS PCR系统专为从所有类型DNA进行最长5 kb片段的高产量和高保真性扩增而设计。它相对于Taq DNA聚合酶具有六倍更高的保真性。
Expand High FidelityPLUS PCR系统也可插入dUTP。因此，可通过在扩增前用热不稳定的尿嘧啶-DNA糖基化酶对含有dUTP的DNA进行预处理来防止交叉污染。 它适用于同时对大量靶标进行扩增。

应用

Expand™ High FidelityPLUS PCR系统专为从所有类型DNA进行最长5 kb片段的高保真扩增而设计，具有出色的产量、特异性、灵敏度和准确度。该试剂盒是开发新的扩增检测体系或优化现有检测体系的首选产品。
使用Expand High FidelityPLUS PCR系统用于：
  • PCR[1]
  • RT-PCR[2]
  • 引物延伸
  • 防残留

特点和优势

  • 对于防止交叉污染具有保障。
这是进行大量靶标扩增的首选。
  • 可获得六倍更高的保真性和更高的产量，
相对于Taq DNA聚合酶。
  • 高效标记DNA。
使用该试剂盒对DNA进行放射性或非放射性核苷酸标记。

包装

1 个试剂盒包含 4 种组份

质量

使用人基因组DNA、可特异性扩增1.8kb PCR产物的EPO引物以及可特异性扩增4.8kb PCR产物的tPA引物对每个批次进行PCR功能检测。

单位定义

体积活力：5 U/μL

其他说明

仅用于生命科学研究。不可用于诊断。

法律信息

有机合成中，过渡金属催化的交叉偶联反应是构建C-C键、C-N键和C-O键的有效途径。研究人员致力于开发出最有效的配体，提高催化剂性能。在稳定和活化中心金属原子以及微调转化选择性方面，配体发挥关键作用。事实证明，膦是交叉偶联反应中最重要的配体，Negishi、Suzuki、Stille、Heck、Sonogashira和Buchwald-Hartwig等交叉偶联反应都需要膦的参与。
Expand is a trademark of Roche

仅试剂盒组分

产品编号
说明
  • Expand High FidelityPLUS Enzyme Blend 5 U/μl
  • Expand High FidelityPLUS Reaction Buffer, with 7.5 mM MgCl2 5x concentrated
  • Expand High FidelityPLUS Reaction Buffer, without MgCl2 5x concentrated
  • MgCl2 Stock Solution 25 mM

危险声明

H412

预防措施声明

P273 - P501

危险分类

Aquatic Chronic 3

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

does not flash

闪点(°C)

does not flash

法规信息

常规特殊物品

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    14889837
    14889830
    14889823
    12335731
    14889834

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