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504971-40

Supelco

Discovery® C18 (5 µm) HPLC Columns

L × I.D. 25 cm × 4 mm, HPLC Column

Synonym(s):

C18 TM=["Discovery"] HPLC Column

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Select a Size

5 G
CN¥870.17
25 G
CN¥3,624.03
100 G
CN¥12,155.56

CN¥870.17

Please contact Customer Service for Availability
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5 G
CN¥870.17
25 G
CN¥3,624.03
100 G
CN¥12,155.56

About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

CN¥870.17

Please contact Customer Service for Availability

Product Name

Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm

material

stainless steel column

Agency

suitable for USP L1

product line

Discovery®

feature

endcapped

manufacturer/tradename

Discovery®

packaging

1 ea of

extent of labeling

12% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

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This Item
N5538SAB4300426N4142
抗硝基酪氨酸 兔抗 affinity isolated antibody, buffered aqueous solution

N0409

抗硝基酪氨酸 兔抗

单克隆抗-3-硝基酪氨酸 小鼠抗 clone 18G4, purified immunoglobulin, buffered aqueous glycerol solution

N5538

单克隆抗-3-硝基酪氨酸 小鼠抗

Anti-NOS1 (Ab-852) antibody produced in rabbit affinity isolated antibody

SAB4300426

Anti-NOS1 (Ab-852) antibody produced in rabbit

抗-神经丝200 兔抗 IgG fraction of antiserum, buffered aqueous solution

N4142

抗-神经丝200 兔抗

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

Quality Level

200

Quality Level

-

Quality Level

100

Quality Level

200

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

Gene Information

human ... NOS1(4842)

Gene Information

human ... NOS1(4842)

Gene Information

human ... NOS1(4842)

Gene Information

human ... NEFH(4744)
mouse ... Nefh(380684)
rat ... Nefh(24587)

technique(s)

immunohistochemistry (frozen sections): 1:1000 using methacarn-fixed, frozen sections of human Alzheimer′s Disease brain, indirect immunofluorescence: 1:1,000 using A549 cells metabolically labeled with 3-nitrotyrosine, western blot: 1:1,000 using nitrated-BSA, indirect ELISA: 1:1,000 using nitrated-BSA, microarray: suitable

technique(s)

western blot: 1:1,000 using mouse and rat brain

technique(s)

indirect immunofluorescence: 1:100-1:200

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80 using animal cerebellum sections, microarray: suitable, western blot: suitable

General description

Use Discovery® C18 for any method that specifies a C18. The exceptional peak shape, reproducibility, and stability make it the column of choice for all C18 methods from demanding to routine.

Application


  • Identification, isolation and characterization of dolutegravir forced degradation products and their cytotoxicity potential.: Utilizes the Discovery® C18 HPLC Column to study the stability and degradation of the antiretroviral drug dolutegravir, crucial for ensuring drug safety and efficacy (Saida et al., 2019).

  • Rapid, Refined, and Robust Method for Expression, Purification, and Characterization of Recombinant Human Amyloid beta 1-42.: This methodological study leverages the Discovery® C18 HPLC Column for the purification and analysis of amyloid beta peptides, contributing to research on Alzheimer′s disease (Prakash et al., 2019).

  • Development and validation of a HPLC electrochemical detection method to measure COMT activity as a tool in drug development.: Showcases the use of the Discovery® C18 HPLC Column in developing a novel analytical method for measuring catechol-O-methyltransferase (COMT) activity, instrumental in the development of psychiatric and neurological medications (Viljoen et al., 2019).

Features and Benefits

  • Excellent reproducibility
  • Exceptional peak shape for basic and acidic analytes
  • Stable, low-bleed LC-MS separations
  • Separation of peptides and small proteins
  • Lower hydrophobicity than many comparable C18 columns, providing faster analysis

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    293818-06
    293818-05
    293818-04
    293818-03
    293818-02

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    Find documentation for the products that you have recently purchased in the Document Library.

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    T Nagano et al.
    Journal of dental research, 88(9), 823-828 (2009-09-22)
    Mmp-20 and Klk4 are the two key enamel proteases. Can both enzymes process amelogenin to generate the major cleavage products that accumulate during the secretory stage of amelogenesis? We isolated Mmp-20 and Klk4 from developing pig teeth and used them
    R Nageswara Rao et al.
    Biomedical chromatography : BMC, 23(11), 1145-1150 (2009-05-16)
    A highly sensitive and selective on-line two-dimensional reversed-phase liquid chromatography/electrospray ionization-tandem mass spectrometry (2D-LC-ESI/MS/MS) method was developed and validated to determine rifaximin in rat serum by direct injection. The 2D-LC-ESI/MS/MS system consisted of a restricted access media column for trapping
    Pei Yuan et al.
    Chemistry (Weinheim an der Bergstrasse, Germany), 15(42), 11319-11325 (2009-09-18)
    This study systematically investigates periodic mesoporous organosilicas (PMOs) with controlled helical and concentric circular (CC) pore architectures prepared through a basic-catalyzed sol-gel process by using an achiral cationic surfactant trimethyloctadecylammonium bromide (C(18)TAB) as a structure-directing agent, perfluorooctanoic acid (PFOA) as
    Yoshinori Masukawa et al.
    Journal of lipid research, 49(7), 1466-1476 (2008-03-25)
    Ceramides (CERs) in human stratum corneum (SC) play physicochemical roles in determining barrier and water-holding functions of the skin, and specific species might be closely related to the regulation of keratinization, together with other CER-related lipids. Structures of those diverse
    Pilar Heredia-Middleton et al.
    Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 149(4), 552-556 (2008-02-01)
    Heat shock protein 70 (HSP70, 70 kDa) is the most commonly expressed protein in response to thermal stress. The extent of its expression is associated with differences in environmental temperatures. We investigated the heat shock response in red blood cells
    View All Related Papers

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