Skip to Content

Dear Customer:

The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

Merck
CN
All Photos(4)

Key Documents

View All Documentation

Safety Information

N0409

Sigma-Aldrich

Anti-Nitrotyrosine antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Nitrotyrosine Antibody, Nitrotyrosine Antibody - Anti-Nitrotyrosine antibody produced in rabbit

Sign Into View Organizational & Contract Pricing

Select a Size

25 μL
¥2,125.86
0.2 ML
¥5,187.66

¥2,125.86

Please contact Customer Service for Availability
Request a Bulk Order
All Photos(4)

Select a Size

Change View
25 μL
¥2,125.86
0.2 ML
¥5,187.66

About This Item

MDL number:
MFCD01322409
UNSPSC Code:
12352203
NACRES:
NA.41

¥2,125.86

Please contact Customer Service for Availability
Request a Bulk Order

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

wide range

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry (frozen sections): 1:1000 using methacarn-fixed, frozen sections of human Alzheimer′s Disease brain
indirect ELISA: 1:1,000 using nitrated-BSA
indirect immunofluorescence: 1:1,000 using A549 cells metabolically labeled with 3-nitrotyrosine
microarray: suitable
western blot: 1:1,000 using nitrated-BSA

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
N5538SAB4300426N4142
抗硝基酪氨酸 兔抗 affinity isolated antibody, buffered aqueous solution

N0409

抗硝基酪氨酸 兔抗

单克隆抗-3-硝基酪氨酸 小鼠抗 clone 18G4, purified immunoglobulin, buffered aqueous glycerol solution

N5538

单克隆抗-3-硝基酪氨酸 小鼠抗

Anti-NOS1 (Ab-852) antibody produced in rabbit affinity isolated antibody

SAB4300426

Anti-NOS1 (Ab-852) antibody produced in rabbit

抗-神经丝200 兔抗 IgG fraction of antiserum, buffered aqueous solution

N4142

抗-神经丝200 兔抗

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

Quality Level

200

Quality Level

-

Quality Level

100

Quality Level

200

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

Gene Information

human ... NOS1(4842)

Gene Information

human ... NOS1(4842)

Gene Information

human ... NOS1(4842)

Gene Information

human ... NEFH(4744)
mouse ... Nefh(380684)
rat ... Nefh(24587)

technique(s)

immunohistochemistry (frozen sections): 1:1000 using methacarn-fixed, frozen sections of human Alzheimer′s Disease brain, indirect immunofluorescence: 1:1,000 using A549 cells metabolically labeled with 3-nitrotyrosine, western blot: 1:1,000 using nitrated-BSA, indirect ELISA: 1:1,000 using nitrated-BSA, microarray: suitable

technique(s)

western blot: 1:1,000 using mouse and rat brain

technique(s)

indirect immunofluorescence: 1:100-1:200

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80 using animal cerebellum sections, microarray: suitable, western blot: suitable

General description

Peroxinitrite (ONOO-), a potent oxidant linked to cellular oxidative damage, is an important member of the family of reactive oxygen and nitrogen species, rapidly generated in vivo from nitric oxide and superoxide anion. Nitrotyrosine is a product of tyrosine nitration, that is facilitated by reactive nitrogen species.

Specificity

Anti-Nitrotyrosine recognizes nitrated proteins and 3-nitro-L-tyrosine. It does not cross-react with L-tyrosine, ρ-nitro-L-phenylalanine, 3-amino-L-tyrosine, 3-chloro-L-tyrosine and phospho-L-tyrosine BSA conjugates.
Reacts with peroxynitrite-nitrated proteins and 3-nitro-L-tyrosine. Does not crossreact with L-tyrosine, p-nitro-L-phenylalanine, 3-amino-L-tyrosine, 3-chloro-L-tyrosine, and phospho-L-tyrosine. Binding of the antibody is blocked by 3-nitrotyrosine.

Immunogen

nitrated KLH (Keyhole Limpet Hemocyanin).

Application

Anti-Nitrotyrosine antibody produced in rabbit has been used in:
  • immunoblotting
  • enzyme-linked immunosorbent assay (ELISA)
  • immunohistochemistry
  • immunofluorescence immunocytochemistry
  • confocal microscopy
  • immunofluorescent staining

Biochem/physiol Actions

Peroxynitrite has been shown to promote the nitration of tyrosine residues in proteins, and oxidation of redox metal centers, DNA, lipids and cysteine and methionine residues in proteins, suggesting that it plays a major role in oxidative cellular damage. Peroxynitrite has been implicated in the pathogenesis of several inflammatory, infectious and degenerative human disease, including neurological conditions such as Alzheimer′s disease, amyotrophic lateral sclerosis (ALS), in the pathogenesis of asthma, atherosclerosis and a variety of conditions precipitated by endothelial injury.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Purified by affinity chromatography on a column of 3-nitro-L-tyrosine.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品
含少量动物源组分生物产品
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000121450
    0000175138
    0000239420
    0000094431
    0000111595

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Endothelial or vascular smooth muscle cell-specific expression of human NOX5 exacerbates renal inflammation, fibrosis and albuminuria in the Akita mouse
    Jha JC, et al.
    Diabetologia, 62(9), 1712-1726 (2019)
    Parkinson's disease transgenic mitochondrial cybrids generate Lewy inclusion bodies
    Trimmer PA, et al.
    Journal of Neurochemistry, 88(4), 800-812 (2004)
    Andreas Warnecke et al.
    Journal of neuroimmunology, 303, 1-12 (2016-12-25)
    Post-translational modifications of autoantigens are hypothesized to affect their immunogenicity. We here report that nitration of tyrosine 40 in Myelin Oligodendrocyte Glycoprotein (MOG) abrogates its encephalitogenicity both at protein and peptide levels in the experimental autoimmune encephalomyelitis (EAE) model in
    Ru-Jeng Teng et al.
    American journal of physiology. Cell physiology, 310(1), C80-C88 (2015-10-23)
    Nitration of both protein-bound and free tyrosine by reactive nitrogen species results in the formation of nitrotyrosine (NT). We previously reported that free NT impairs microtubule polymerization and uncouples endothelial nitric oxide synthase (eNOS) function in pulmonary artery endothelial cells
    Lipopolysaccharide Induces Subacute Cerebral Microhemorrhages with Involvement of Nitric Oxide Synthase in Rats
    Zeng J, et al.
    Journal of Stroke and Cerebrovascular Diseases : The Official Journal of National Stroke Association, 27(7), 1905-1913 (2018)
    View All Related Papers

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service