N0409
Anti-Nitrotyrosine antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
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Synonym(s):
Nitrotyrosine Antibody, Nitrotyrosine Antibody - Anti-Nitrotyrosine antibody produced in rabbit
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
wide range
packaging
antibody small pack of 25 μL
technique(s)
immunohistochemistry (frozen sections): 1:1000 using methacarn-fixed, frozen sections of human Alzheimer′s Disease brain
indirect ELISA: 1:1,000 using nitrated-BSA
indirect immunofluorescence: 1:1,000 using A549 cells metabolically labeled with 3-nitrotyrosine
microarray: suitable
western blot: 1:1,000 using nitrated-BSA
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NOS1(4842)
General description
Peroxinitrite (ONOO-), a potent oxidant linked to cellular oxidative damage, is an important member of the family of reactive oxygen and nitrogen species, rapidly generated in vivo from nitric oxide and superoxide anion. Nitrotyrosine is a product of tyrosine nitration, that is facilitated by reactive nitrogen species.
Specificity
Anti-Nitrotyrosine recognizes nitrated proteins and 3-nitro-L-tyrosine. It does not cross-react with L-tyrosine, ρ-nitro-L-phenylalanine, 3-amino-L-tyrosine, 3-chloro-L-tyrosine and phospho-L-tyrosine BSA conjugates.
Reacts with peroxynitrite-nitrated proteins and 3-nitro-L-tyrosine. Does not crossreact with L-tyrosine, p-nitro-L-phenylalanine, 3-amino-L-tyrosine, 3-chloro-L-tyrosine, and phospho-L-tyrosine. Binding of the antibody is blocked by 3-nitrotyrosine.
Immunogen
nitrated KLH (Keyhole Limpet Hemocyanin).
Application
Anti-Nitrotyrosine antibody produced in rabbit has been used in:
- immunoblotting
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemistry
- immunofluorescence immunocytochemistry
- confocal microscopy
- immunofluorescent staining
Biochem/physiol Actions
Peroxynitrite has been shown to promote the nitration of tyrosine residues in proteins, and oxidation of redox metal centers, DNA, lipids and cysteine and methionine residues in proteins, suggesting that it plays a major role in oxidative cellular damage. Peroxynitrite has been implicated in the pathogenesis of several inflammatory, infectious and degenerative human disease, including neurological conditions such as Alzheimer′s disease, amyotrophic lateral sclerosis (ALS), in the pathogenesis of asthma, atherosclerosis and a variety of conditions precipitated by endothelial injury.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Preparation Note
Purified by affinity chromatography on a column of 3-nitro-L-tyrosine.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
含少量动物源组分生物产品
Certificates of Analysis (COA)
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Parkinson's disease transgenic mitochondrial cybrids generate Lewy inclusion bodies
Trimmer PA, et al.
Journal of Neurochemistry, 88(4), 800-812 (2004)
Lipopolysaccharide Induces Subacute Cerebral Microhemorrhages with Involvement of Nitric Oxide Synthase in Rats
Zeng J, et al.
Journal of Stroke and Cerebrovascular Diseases : The Official Journal of National Stroke Association, 27(7), 1905-1913 (2018)
NTyroSite: Computational Identification of Protein Nitrotyrosine Sites Using Sequence Evolutionary Features
Hasan Md M, et al.
Molecules (Basel), 23(7) (2018)
Constantino Tomas-Sanchez et al.
Journal of immunology research, 2016, 4039837-4039837 (2016-09-17)
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