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Key Documents

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T9650

Sigma-Aldrich

Tris Acetate-EDTA buffer

10× concentrate, BioReagent, non-sterile; 0.2 μm filtered

Synonym(s):

TAE buffer

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5 ML
CN¥1,446.06
15 ML
CN¥3,109.05

CN¥1,446.06

Estimated to ship onJuly 21, 2025Details

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5 ML
CN¥1,446.06
15 ML
CN¥3,109.05

About This Item

MDL number:
MFCD00236357
UNSPSC Code:
41105319
PubChem Substance ID:
329826908
NACRES:
NA.25

CN¥1,446.06

Estimated to ship onJuly 21, 2025Details

Request a Bulk Order

grade

Molecular Biology

Quality Level

200

sterility

non-sterile; 0.2 μm filtered

product line

BioReagent

form

solution

suitability

suitable for gel electrophoresis (after dilution to working concentration)

foreign activity

Protease, none detected
RNAse, none detected

SMILES string

CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)

InChI key

HGEVZDLYZYVYHD-UHFFFAOYSA-N

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P7892

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matrix active group

N-hydroxysuccinimide active ester

matrix active group

cyanate or related structures

matrix active group

-

matrix active group

-

form

isopropanol suspension

form

lyophilized powder

form

suspension

form

-

technique(s)

protein array: suitable

technique(s)

affinity chromatography: suitable

technique(s)

-

technique(s)

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

Application

TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Tris Acetate-EDTA buffer has been used for the preparation of agarose gel during DNA agarose gel electrophoresis.[1][2]

Other Notes

0.4 M Tris acetate, pH approx. 8.3, containing 0.01 M EDTA.

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).
Solution prepared with 18 megohm water

Pictograms

Health hazard
GHS08

Signal Word

Warning

Hazard Statements

H373

Precautionary Statements

P260 - P313 - P501

Hazard Classifications

STOT RE 2 Inhalation

Target Organs

Respiratory Tract

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCP3732
    0000318838
    0000318837
    0000310651
    SLCP3732

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    Find documentation for the products that you have recently purchased in the Document Library.

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    Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries
    Quan J and Tian J
    Nature Protocols, 6(2), 242-242 (2011)
    Yuksel Temiz et al.
    Scientific reports, 8(1), 10603-10603 (2018-07-15)
    The ever-increasing need for portable, easy-to-use, cost-effective, and connected point-of-care diagnostics (POCD) has been one of the main drivers of recent research on lab-on-a-chip (LoC) devices. A majority of these devices use microfluidics to manipulate precisely samples and reagents for
    Joseph P Torella et al.
    Nature protocols, 9(9), 2075-2089 (2014-08-08)
    Recombination-based DNA construction methods, such as Gibson assembly, have made it possible to easily and simultaneously assemble multiple DNA parts, and they hold promise for the development and optimization of metabolic pathways and functional genetic circuits. Over time, however, these

    Protocols

    TAE and TBE Running Buffers Recipe & Video

    TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service