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Sigma-Aldrich

TAE Buffer, 10X, Molecular Biology Grade

A 10X concentrate that can be diluted to a 1X solution containing 40 mM Tris, 40 mM acetate, and 1 mM EDTA, pH ~8.3.

Synonym(s):

Tris-Acetate-EDTA Buffer

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

technique(s)

electrophoresis: suitable

foreign activity

DNase, RNase, protease, none detected

shipped in

ambient

storage temp.

15-25°C

General description

Tris, acetate, and ethylenediaminetetraacetic acid (TAE) buffer consists of tris(2-amino-2-[hydroxymethyl]-1,3-propanediol) base, acetate, and ethylenediaminetetraacetic acid (EDTA). This is one of the most used running buffers in nucleic acid electrophoresis. TAE buffer improves the separation or resolution of large DNA fragments.TAE, a standard buffer, is mainly used to perform agarose gel electrophoresis of DNA and RNA in slab gels. TAE is one of the commonly used buffers in every biochemistry and molecular biology laboratory.

Application

TAE Buffer, 10X, Molecular Biology Grade - Calbiochem has been used in 1X concentration for the preparation of agarose gel and as a running buffer in gel electrophoresis.

Warning

Toxicity: Irritant (B)

Preparation Note

Dilute 1:10 with H₂O to prepare a 1X solution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Bin Li et al.
Nature protocols, 13(5), 899-914 (2018-04-07)
Cpf1, a CRISPR endonuclease discovered in Prevotella and Francisella 1 bacteria, offers an alternative platform for CRISPR-based genome editing beyond the commonly used CRISPR-Cas9 system originally discovered in Streptococcus pyogenes. This protocol enables the design of engineered CRISPR-Cpf1 components, both
Modification of gel architecture and TBE/TAE buffer composition to minimize heating during agarose gel electrophoresis
Sanderson BA, et al.
Analytical Biochemistry, 454, 44-52 (2014)
Brian A Sanderson et al.
Analytical biochemistry, 454, 44-52 (2014-03-19)
Agarose gel electrophoresis of DNA and RNA is routinely performed using buffers containing either Tris, acetate, and EDTA (TAE) or Tris, borate, and EDTA (TBE). Gels are run at a low, constant voltage (∼10 V/cm) to minimize current and asymmetric

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