T4415
Tris-Borate-EDTA buffer
BioReagent, suitable for electrophoresis, 10× concentrate
Synonym(s):
TBE buffer
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About This Item
Recommended Products
Quality Level
product line
BioReagent
form
solution
impurities
DNase and RNase, none detected
suitability
suitable for electrophoresis
SMILES string
OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
InChI key
OSBLTNPMIGYQGY-UHFFFAOYSA-N
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This Item | 21223 | 391947 | 900195 |
|---|---|---|---|
| form powder | form powder | form solid | form powder |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| description Particle size: <5.0 μm ± 1.0 μm | description - | description - | description Particle size: <2.5 μm± 0.5 μm, Total Heavy Metals: ≤20 ppm |
| surface area ≥80 m2/g | surface area - | surface area - | surface area ≥80 m2/g |
| particle size 5 μm | particle size - | particle size - | particle size - |
General description
Application
Tris-Borate-EDTA buffer has been used for pulsed-field gel electrophoresis (PFGE).[3]
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Packaging
Preparation Note
Other Notes
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Repr. 1B
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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Protocols
TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.
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