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Key Documents

Safety Information

P4649

Sigma-Aldrich

Phosphorylase b from rabbit muscle

For use as a marker in SDS-PAGE

Synonym(s):

α-Glucan Phosphorylase, 1,4-α-D-Glucan:orthophosphate α-D-glucosyltransferase, Glycogen Phosphorylase

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1 G
¥1,617.24
5 G
¥7,340.77

¥1,617.24


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1 G
¥1,617.24
5 G
¥7,340.77

About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

¥1,617.24


Please contact Customer Service for Availability

Request a Bulk Order

Quality Level

form

powder

mol wt

observed mol wt ~97 kDa

packaging

vial of 0.5 mg

storage temp.

2-8°C

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Show Differences

1 of 4

This Item
P1261P6635SRE0069
form

powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

observed mol wt ~97 kDa

mol wt

-

mol wt

97,200 Da by calculation

mol wt

-

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

packaging

vial of 0.5 mg

packaging

-

packaging

-

packaging

-

General description

Phosphorylase b is a dimer, usually exists in inactive form in the skeletal muscles.[1][2] Equilibrium exists between an active relaxed (R) state and a less active tense (T). Phosphorylase b favors the T state.[3] The enzyme possesses three domains, the N-terminal domain, glycogen-binding domain and the C-terminal domain.[1]

Application

Phosphorylase b from rabbit muscle has been used as a molecular weight marker in 12% polyacrylamide gel for keratinase,[4] and stress-70 protein.[5]
Phosphorylase b from rabbit muscle is to be used as a marker in SDS-PAGE. Phosphorylase b is used during chemical cross-linking studies as a SDS-PAGE molecular weight standard[6][7].

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物源性产品

  • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

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    1. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    2. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    3. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    4. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    5. Has the amino acid sequence of phosphorylase from rabbit muscle been published?

      The sequence has been published. The amino acid composition is:Asp5l, Asn45, Thr35, Ser29, Glu64, Gln31, Pro36, Gly48, Ala63, Cys9, Val62, Met2l, Ile49, Leu79, Tyr36, Phe38, Trp12, Lys48, HiS22, and Arg63. Reference for the sequence and amino acid composition: K. Titani et al., Proc. Nat. Acad. Sci., USA, 74, 4762-4766 (1977) (see attached pdf).Product P1261 is phosphorylase a, which is the tetrameric form. Products P4649 and P6635 are phosphorylase b, the dimeric  form. The sequence information of the subunit would be the same for both forms.

    6. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

    Signal Transduction and Protein Phosphorylation (2012)
    Stress-70 proteins in marine mussel Mytilus galloprovincialis as biomarkers of environmental pollution: a field study
    Hamer B, et al.
    Environment International, 30(7), 873-882 (2004)
    Crystallographic studies on the activity of glycogen phosphorylase b
    Weber IT, et al.
    Nature, 274(5670), 433-433 (1978)
    Characterization of alkaline keratinase of Bacillus licheniformis strain HK-1 from poultry waste
    Korkmaz H, et al.
    Annales de Microbiologie, 54(2), 201-211 (2004)
    B Chen et al.
    The Journal of biological chemistry, 275(45), 34946-34953 (2000-08-17)
    The envelope glycoprotein, gp160, of simian immunodeficiency virus (SIV) shares approximately 25% sequence identity with gp160 from the human immunodeficiency virus, type I, indicating a close structural similarity. As a result of binding to cell surface CD4 and co-receptor (e.g.

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