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The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

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Key Documents

Safety Information

MOD50

Sigma-Aldrich

Imprint® DNA Modification Kit

For bisulfite DNA conversion & purification

Synonym(s):

Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification

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Select a Size

25 G
CN¥361.44
100 G
CN¥1,440.54

CN¥361.44

Please contact Customer Service for Availability
Request a Bulk Order
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25 G
CN¥361.44
100 G
CN¥1,440.54

About This Item

EC Number:
231-548-0
UNSPSC Code:
41116158
NACRES:
NA.54

CN¥361.44

Please contact Customer Service for Availability
Request a Bulk Order

Quality Level

200

usage

sufficient for 50 reactions

storage temp.

20-25°C

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Retinoblastoma protein human recombinant, expressed in insect cells, ≥70% (SDS-PAGE)

SRP2081

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RB Active human recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

SRP0256

RB Active human

Anti-phospho-Retinoblastoma (pSer807) antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

R3778

Anti-phospho-Retinoblastoma (pSer807) antibody produced in rabbit

Anti-Phospho-Rb-S807/811 antibody produced in rabbit

SAB5700366

Anti-Phospho-Rb-S807/811 antibody produced in rabbit

assay

≥70% (SDS-PAGE)

assay

≥80% (SDS-PAGE)

assay

-

assay

-

biological source

human

biological source

human

biological source

rabbit

biological source

rabbit

recombinant

expressed in insect cells

recombinant

expressed in E. coli

recombinant

-

recombinant

-

concentration

400 μg/mL

concentration

>0.02 mg/mL

concentration

-

concentration

4.05 mg/ml

form

frozen liquid

form

aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

~107.5 kDa

mol wt

43.5 kDa

mol wt

-

mol wt

106

General description

The Imprint® DNA Modification Kit contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. Converted DNA is suitable for a variety of downstream applications including Methylation-Specifc PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.

Application

Imprint® DNA Modification Kit has been used for bisulfite modification of DNA.[1][2][3]

Features and Benefits

  • Only 50 picograms of DNA or 20 cells are required
  • Procedure takes less than 2 hours
  • Greater than 99% conversion rate
  • Extremely low degradation
  • Option of convenient one-step protocol
  • Consistent and reproducible Bisulfite Modification
  • Can be used with genomic, endonuclease digested, and FFPE DNA

Storage and Stability

All components can be stored at room temperature. Each vial of DNA Modification Powder is sufficient for ten DNA modifications. Once dissolved, the solution can be stored at -20 °C for one week, kept away from light. Before use, the frozen solution must be thawed at room temperature and vortexed for two minutes.

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS
  • T3566Clear-view™ Snap-Cap microtubes, size 1.5 mL, naturalSDS

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H290,H302,H314,H412

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

Supplementary Hazards

EUH031

Storage Class Code

8B - Non-combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

危险化学品
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000333642
    0000333642
    SLCQ0447
    SLCD4557
    SLCD4559

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    1. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    2. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    3. How many reactions can be run with Product MOD50, Imprint® DNA Modification Kit?

      This kit can be used for 50 reactions.

    4. What is the minimum amount of DNA that can be used with Product MOD50, Imprint® DNA Modification Kit?

      DNA amounts ranging from 0.1 ng to 1 mg have been used successfully. For optimal modification, the DNA quantity should be in the range of 50-200 ng.

    5. Can I use Product MOD50, Imprint® DNA Modification Kit with DNA templates that contain high GC content or secondary structures?

      Yes, but it will be necessary to increase the bisulfite reaction time to 150 to 180 min.

    6. Can you store the DNA modified using Product MOD50, Imprint® DNA Modification Kit?

      The modified DNA generated with this kit can be stored for 2 months at -20 °C, 6 months at -80 °C.

    7. Can Product MOD50, Imprint® DNA Modification Kit, be used on formalin-fixed tissue ?

      Yes, it can be used on formalin-fixed tissue, but one would need to amplify the gene of interest before bisulfite treatment

    8. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    9. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    10. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

    The elephant shark methylome reveals conservation of epigenetic regulation across jawed vertebrates
    Peat JR, et al.
    F1000Research (2017)
    Julian R Peat et al.
    Methods in molecular biology (Clifton, N.J.), 1708, 161-169 (2017-12-11)
    The epigenetic mark 5-methylcytosine confers heritable regulation of gene expression that can be dynamically modulated during transitions in cell fate. With the development of high-throughput sequencing technologies, it is now possible to obtain comprehensive genome-wide maps of the mammalian DNA
    Sarah Rose Huffman et al.
    Methods in molecular biology (Clifton, N.J.), 925, 201-209 (2012-08-22)
    Researchers whose experimental models are mammalian oocytes and preimplantation embryos are often limited by the yield of nucleic acids that can be isolated from such a small sample size. In addition, the limited number of cells from these types of
    Javed Hussain Choudhury et al.
    Methods in molecular biology (Clifton, N.J.), 1726, 111-122 (2018-02-23)
    DNA methylation plays a decisive role in the regulation and control of gene expression. DNA methylation is a covalent modification, in which a methyl group is attached to the 5th carbon of the cytosine ring of a CpG dinucleotide that
    MicroRNA-20 induces methylation of hepatitis B virus covalently closed circular DNA in human hepatoma cells
    In Young Moon, et al.
    Molecular Medicine Reports, 2285-2293 (2019)
    View All Related Papers

    Articles

    DNA Methylation & Bisulfite Conversion

    Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.

    Protocols

    ChIP-qPCR Data Analysis

    Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

    Related Content

    Imprint ® DNA Modification Kit (MOD50) Protocol

    The Imprint DNA Modification Kit provides the reagents needed for bisulfite conversion and post-modification clean-up of DNA samples in less than 2 hours.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service