CHP1
Imprint® Chromatin Immunoprecipitation Kit
Complete ChIP reaction in 6 hours in flexible strip well format
Synonym(s):
Chromatin Immunoprecipitation
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About This Item
UNSPSC Code:
41116158
NACRES:
NA.54
Quality Level
storage temp.
−20°C
Related Categories
General description
Imprint Chromatin Immunoprecipitation Kit provides a complete solution for Chromatin Immunoprecipitation including columns and reagents for DNA purification and an integrated protocol for ChIP DNA amplification with our GenomePlex® Whole Genome Amplification Kit (WGA2) . The flexible format allows for immunoprecipitation and purification of DNA from mammalian cells or tissue in a convenient strip-well format.
For Frequently Asked Questions about this kit, please see ChIP Troubleshooting Questions.
For Frequently Asked Questions about this kit, please see ChIP Troubleshooting Questions.
Application
The Imprint Chromatin Immunoprecipitation Kit is a flexible, rapid and complete method for investigating protein-DNA interactions by chromatin immunoprecipitation (ChIP).
- Suitable for downstream applications
- Individual target characterization to genome-wide profiling techniques
- Characterization of signal transduction pathways
- Verification of ChIp-chIP and ChIP-seq data
Features and Benefits
- Fast—Total protocol time of less than 6 hours making the Imprint kit the fastest on the market
- Sensitive—As few as 10,000 cells required for each ChIP sample
- Convenient—Fewest steps of any available ChIP protocol
- Flexible—Protocols for cells or tissue, and convenient strip-well format for high-throughput applications
- Complete—Includes columns and reagents for DNA purification as well as an integrated protocol for amplification with the GenomePlex technology
Legal Information
GenomePlex is a registered trademark of Takara Bio USA, Inc.
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Kit Components Also Available Separately
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Description
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Central nervous system
Storage Class Code
3 - Flammable liquids
Flash Point(F)
53.6 °F - closed cup
Flash Point(C)
12 °C - closed cup
Regulatory Information
监管及禁止进口产品
Certificates of Analysis (COA)
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Poulomi Banerjee et al.
Journal of molecular medicine (Berlin, Germany), 94(12), 1397-1409 (2016-08-19)
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Ghazala Begum et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 26(4), 1694-1703 (2012-01-10)
Undernutrition during pregnancy is implicated in the programming of offspring for the development of obesity and diabetes. We hypothesized that maternal programming causes epigenetic changes in fetal hypothalamic pathways regulating metabolism. This study used sheep to examine the effect of
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Nucleic acids research, 41(6), 3699-3712 (2013-02-12)
Stem cell fate decisions are controlled by a molecular network in which transcription factors and miRNAs are of key importance. To systemically investigate their impact on neural stem cell (NSC) maintenance and neuronal commitment, we performed a high-throughput mRNA and
Lucie Hyrsova et al.
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The organic cation transporter 1 (OCT1) transports cationic drugs into hepatocytes. The high hepatic expression of OCT1 is controlled by the HNF4α and USF transcription factors. Pregnane X receptor (PXR) mediates induction of the principal xenobiotic metabolizing enzymes and transporters
Moonmoon Deb et al.
Gene, 581(1), 75-84 (2016-01-23)
Caveolin-1 (CAV1) is an important structural component of cellular caveolae involved in cell signaling. CAV1 gene on/off regulatory mechanism in multiple diseases, including cancer is not clearly understood. The tumor suppressor versus oncogene paradox of CAV1 during tumor development tempted
Protocols
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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