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Key Documents

Safety Information

SRP0256

Sigma-Aldrich

RB Active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonym(s):

OSRC, RB1, Retinoblastoma 1, Retinoblastoma-associated protein, p105-Rb

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25 ML
CN¥226.18
1 L
CN¥971.51
4 L
CN¥2,281.90

CN¥226.18

Available to ship onApril 27, 2025Details

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25 ML
CN¥226.18
1 L
CN¥971.51
4 L
CN¥2,281.90

About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

CN¥226.18

Available to ship onApril 27, 2025Details

Request a Bulk Order

biological source

human

recombinant

expressed in E. coli

Assay

≥80% (SDS-PAGE)

form

aqueous solution

mol wt

43.5 kDa

packaging

pkg of 200 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

NM_000321

UniProt accession no.

P06400

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volume

4 mL

volume

2 mL

volume

40 mL

volume

15 mL

feature

closure type screw top vial

feature

closure type screw top vial

feature

closure type screw top vial

feature

closure type screw top vial

fitting

thread for 13-425

fitting

thread for 10-425

fitting

thread for 24-400

fitting

thread for 18-400

material

amber glass vial

material

amber glass vial

material

amber glass vial

material

amber glass vial

packaging

pkg of 100 ea

packaging

pkg of 100 ea

packaging

pkg of 100 ea

packaging

pkg of 100 ea

O.D. × H × I.D.

15 mm × 45 mm × 8 mm

O.D. × H × I.D.

12 mm × 32 mm × 6 mm

O.D. × H × I.D.

29 mm × 82 mm × 17 mm

O.D. × H × I.D.

21 mm × 70 mm × 12 mm

General description

The gene encoding Retinoblastoma-associated protein (RB1) is a tumor suppressor gene localized on human chromosome 13q14.2.[1] Human recombinant RB protein (773-end) with N-terminal GST tag (GenBank Accession No. NM_000321), expressed in a E. coli expression system.

Application

Retinoblastoma-associated protein (RB1) has been used in cell activity assay.[2] It is also used as a kinase substrate for the kinase assay and in Western blotting.

Biochem/physiol Actions

Retinoblastoma-associated protein (RB1) modulates the cell cycle. It acts as a repressor of transcription mediated by E2 factor (E2F) and influences transcriptional silencing. RB1 modulates mitochondrial activities and has a role in the organisation of heterochromatin. Mutations in the gene encoding this protein have been associated with childhood retinoblastoma.[3][4][5]

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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