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DNAQF

Sigma-Aldrich

DNA Quantitation Kit, Fluorescence Assay

Quantitation of DNA using bisBenzimide

Synonym(s):

dsDNA quantification

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About This Item

UNSPSC Code:
41105500
NACRES:
NA.84

Quality Level

200

usage

sufficient for 750 assays (2 ml each)

technique(s)

nucleic acid detection: suitable

storage temp.

−20°C

General description

The kit has all the reagents necessary for quantitation of DNA using the fluorescent dye bisBenzimide (Hoechst 33258). The kit allows highly sensitive, quick, and accurate determinations of double-stranded DNA from 10ng/ml to 5μg/ml, even in the presence of RNA and proteins. Fluorometry is a highly sensitive and simple method used for DNA quantitation. The fluorescent dye, bisBenzimide H 33258, which binds primarily to AT sequences in the minor groove of double-stranded DNA (dsDNA), is specific for quantitation of nanogram amounts of DNA (10 ng/ml to 10 mg/ml). When excited at 360 nm, the fluorescence emission at 460 nm of the dye increases significantly in the presence of DNA.

Application

DNA Quantitation Kit, Fluorescence Assay has been used to measure the concentration/content of DNA:
  • in neonatal rat ventricular myocytes (NRVMs)
  • in human mesenchymal stem cells (hMSCs)/samples for normalization of the relative alkaline phosphatase (ALP) amount
  • in mouse embryonic fibroblast cell line (NIH3T3 cells)

Features and Benefits

  • Specific for the quantitation of nanogram amounts of DNA
  • Works well with purified preparations as well as with DNA from crude extracts containing RNA and proteins

Kit Components Only

Product No.
Description
  • Detailed technical bulletin 1 ea

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Pricing

Storage Class Code

10 - Combustible liquids

Regulatory Information

动植物源性产品

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Certificates of Analysis (COA)

Lot/Batch Number
0000242664
0000242664
0000177622
0000177622
0000124923

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. I see that one of the componets of Product DNAQF, DNA Quantitation Kit, Fluorescence Assay, is a DNA standard. How is the DNA standard prepared? Can it be purchased separately?  

    The DNA standard (Product No. D4810) used in the kit is a sheared calf thymus DNA. It is the only one tested with the kit and it is only available in the kit. We do not determine the molecular weight.

  6. How can I ensure accurate initial quantification of the DNA standard by spectrophotometric measurement at A260 when using Product DNAQF, DNA Quantitation Kit, Fluorescence Assay?

    The DNA standard (Product No. D4810) should be incubated at 50 °C for 15-30 minutes before determining the concentration spectrophotometrically. This will ensure complete dissolution of the genomic DNA.

  7. What quality of genomic DNA is required for use in Product DNAQF, DNA Quantitation Kit, Fluorescence Assay?

    Genomic DNA purified by phenol chloroform extraction is sufficiently clean for use with this kit.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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