MBD0013
Microbial DNA standard from Escherichia coli
Suitable for PCR, sequencing and NGS, 10 ng/μL
Synonym(s):
E.Coli
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About This Item
NACRES:
NA.51
UNSPSC Code:
41105500
Product Name
Microbial DNA standard from Escherichia coli, Suitable for PCR, sequencing and NGS, 10 ng/μL
form
liquid
concentration
10 ng/μL
technique(s)
DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable
suitability
suitable for restriction endonuclease digests, PCR amplification, Southern blots, and sequencing reactions
shipped in
ambient
storage temp.
−20°C
Quality Level
Related Categories
Application
The genomic DNA is provided at >=10 ng/μL concentration in TE buffer pH 8.0. It is recommended to avoid freeze thaw cycles of this product.
Suitable for Quantitative standard for PCR, Sequencing and NGS
Suitable for Quantitative standard for PCR, Sequencing and NGS
Features and Benefits
- Individual microbial standard for microbiomics and meta-genomics workflow
- Suitable standard for PCR, sequencing and NGS
- Improve Bioinformatics analyses
- Increases reproducibility
- Compare results lab to lab
General description
Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses. Escherichia coli genomic DNA microbial standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs. Escherichia coli is a gram negative, facultative aerobic, rod-shaped coliform bacterium. E. coli colonizes the infant gut within hours of birth and establishes itself as the most abundant facultative anaerobe of the human intestinal microflora for the remainder of life, equipped with the abilities to grow in the ever-changing environment in the gut and cope with the mammalian host interaction. Nevertheless, E. coli can survive in many different ecological habitats, including abiotic environments, and is considered a highly versatile species. Known habitats of E. coli include soil, water, sediment, and food. Some strains of E. coli have evolved and adapted to a pathogenic lifestyle and can cause different disease pathologies.
Read here how to use our standards to ensure data integrity for your microbiome research.
Read here how to use our standards to ensure data integrity for your microbiome research.
Other Notes
Strain: MG1655
Physical form
Liquid - The genomic DNA is provided at >=10 ng/μL concentration in TE buffer pH 8.0
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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James B Kaper et al.
Nature reviews. Microbiology, 2(2), 123-140 (2004-03-26)
Few microorganisms are as versatile as Escherichia coli. An important member of the normal intestinal microflora of humans and other mammals, E. coli has also been widely exploited as a cloning host in recombinant DNA technology. But E. coli is
Order and disorder during Escherichia coli divergence.
Heather Hendrickson
PLoS genetics, 5(1), e1000335-e1000335 (2009-01-24)
Lisa C Crossman et al.
Journal of bacteriology, 192(21), 5822-5831 (2010-08-31)
In most cases, Escherichia coli exists as a harmless commensal organism, but it may on occasion cause intestinal and/or extraintestinal disease. Enterotoxigenic E. coli (ETEC) is the predominant cause of E. coli-mediated diarrhea in the developing world and is responsible
J Paul Brooks et al.
BMC microbiology, 15, 66-66 (2015-04-17)
Characterizing microbial communities via next-generation sequencing is subject to a number of pitfalls involving sample processing. The observed community composition can be a severe distortion of the quantities of bacteria actually present in the microbiome, hampering analysis and threatening the
Articles
DNA standards enhance metagenomics research integrity, offering precise species study and mixed community standards.
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