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Key Documents

Safety Information

MBD0013

Sigma-Aldrich

Microbial DNA standard from Escherichia coli

Suitable for PCR, sequencing and NGS, 10 ng/μL

Synonym(s):

E.Coli

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200 μG
CN¥7,573.10

CN¥7,573.10


Estimated to ship onApril 21, 2025Details

A recombinant, preservative-free antibody is available for your target. Try ZRB1625


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200 μG
CN¥7,573.10

About This Item

UNSPSC Code:
41105500
NACRES:
NA.51

CN¥7,573.10


Estimated to ship onApril 21, 2025Details

A recombinant, preservative-free antibody is available for your target. Try ZRB1625

Quality Level

form

liquid

concentration

10 ng/μL

technique(s)

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

suitability

suitable for restriction endonuclease digests, PCR amplification, Southern blots, and sequencing reactions

shipped in

ambient

storage temp.

−20°C

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1 of 4

This Item
1617316233ABT135
biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

clone

4.47, monoclonal

clone

4.47, monoclonal

clone

4.47, monoclonal

clone

polyclonal

antibody form

purified immunoglobulin

antibody form

-

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

UniProt accession no.

Q05397

UniProt accession no.

Q05397

UniProt accession no.

Q05397

UniProt accession no.

Q05397

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunoprecipitation (IP): suitable

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, western blot: suitable

technique(s)

cell based assay: suitable, western blot: suitable

General description

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses. Escherichia coli genomic DNA microbial standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs. Escherichia coli is a gram negative, facultative aerobic, rod-shaped coliform bacterium. E. coli colonizes the infant gut within hours of birth and establishes itself as the most abundant facultative anaerobe of the human intestinal microflora for the remainder of life, equipped with the abilities to grow in the ever-changing environment in the gut and cope with the mammalian host interaction. Nevertheless, E. coli can survive in many different ecological habitats, including abiotic environments, and is considered a highly versatile species. Known habitats of E. coli include soil, water, sediment, and food. Some strains of E. coli have evolved and adapted to a pathogenic lifestyle and can cause different disease pathologies.


Read here how to use our standards to ensure data integrity for your microbiome research.

Application

The genomic DNA is provided at >=10 ng/μL concentration in TE buffer pH 8.0. It is recommended to avoid freeze thaw cycles of this product.
Suitable for Quantitative standard for PCR, Sequencing and NGS

Features and Benefits

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

Physical form

Liquid - The genomic DNA is provided at >=10 ng/μL concentration in TE buffer pH 8.0

Other Notes

Strain: MG1655

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

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    Find documentation for the products that you have recently purchased in the Document Library.

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    Order and disorder during Escherichia coli divergence.
    Heather Hendrickson
    PLoS genetics, 5(1), e1000335-e1000335 (2009-01-24)
    Lisa C Crossman et al.
    Journal of bacteriology, 192(21), 5822-5831 (2010-08-31)
    In most cases, Escherichia coli exists as a harmless commensal organism, but it may on occasion cause intestinal and/or extraintestinal disease. Enterotoxigenic E. coli (ETEC) is the predominant cause of E. coli-mediated diarrhea in the developing world and is responsible
    James B Kaper et al.
    Nature reviews. Microbiology, 2(2), 123-140 (2004-03-26)
    Few microorganisms are as versatile as Escherichia coli. An important member of the normal intestinal microflora of humans and other mammals, E. coli has also been widely exploited as a cloning host in recombinant DNA technology. But E. coli is
    J Paul Brooks et al.
    BMC microbiology, 15, 66-66 (2015-04-17)
    Characterizing microbial communities via next-generation sequencing is subject to a number of pitfalls involving sample processing. The observed community composition can be a severe distortion of the quantities of bacteria actually present in the microbiome, hampering analysis and threatening the

    Articles

    DNA standards enhance metagenomics research integrity, offering precise species study and mixed community standards.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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