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Key Documents

05-537

Sigma-Aldrich

Anti-FAK Antibody, clone 4.47

clone 4.47, Upstate®, from mouse

Synonym(s):

Anti-FADK, Anti-FADK 1, Anti-FAK, Anti-FAK1, Anti-FRNK, Anti-PPP1R71, Anti-p125FAK, Anti-pp125FAK

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200 μG
¥7,573.10

¥7,573.10

Estimated to ship on2025年4月21日Details

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200 μG
¥7,573.10

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

¥7,573.10

Estimated to ship on2025年4月21日Details

A recombinant, preservative-free antibody is available for your target. Try ZRB1625
Sign In for Best Price
Request a Bulk Order

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4.47, monoclonal

species reactivity

mouse, rat, human

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_005607
NM_153831

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抗 FAK 抗体，克隆 4.47 clone 4.47, Upstate®, from mouse

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抗 FAK 抗体，克隆 4.47

Anti-FAK Antibody, clone 4.47, agarose conjugate clone 4.47, Upstate®, from mouse

16173

Anti-FAK Antibody, clone 4.47, agarose conjugate

Anti-FAK Antibody, clone 4.47, Alexa Fluor™ 488 conjugate clone 4.47, Upstate®, from mouse

16233

Anti-FAK Antibody, clone 4.47, Alexa Fluor™ 488 conjugate

Anti-phospho-FAK (Tyr397) Antibody from rabbit, purified by affinity chromatography

ABT135

Anti-phospho-FAK (Tyr397) Antibody

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

clone

4.47, monoclonal

clone

4.47, monoclonal

clone

4.47, monoclonal

clone

polyclonal

antibody form

purified immunoglobulin

antibody form

-

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

UniProt accession no.

Q05397

UniProt accession no.

Q05397

UniProt accession no.

Q05397

UniProt accession no.

Q05397

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunoprecipitation (IP): suitable

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, western blot: suitable

technique(s)

cell based assay: suitable, western blot: suitable

General description

Focal adhesion kinase (FAK) is a cytoplasmic protein tyrosine kinase that has been implicated to play an important role in integrin-mediated signal transduction pathways. FAK becomes activated and tyrosine phosphorylated in integrin-mediated cell adhesion and is colocalized with integrins and other cytoskeletal proteins in focal contacts in a variety of adherent cells. Physical interactions of FAK with the integrin cytoplasmic domain as well as cytoskeletal proteins talin, paxillin, and/ or tensin have been proposed to play a key role in FAK activation by facilitating its oligomerization and transphosphorylation. FAK shows a rapid increase in tyrosine phosphorylation when stimulated by diverse signaling molecules, including those that regulate embryonic development, cell proliferation, migration and apoptosis, and efficient study methods are in high demand.

Specificity

Recognizes p125FAK; does not cross react with Pyk-2.

Immunogen

GST fusion protein corresponding to residues 1-423 of human FAK (focal adhesion kinase); specific for p125FAK

Application

Anti-FAK Antibody, clone 4.47 is an antibody against FAK for use in IC, IH, IP & WB.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

Quality

Routinely evaluated by immunoblot on 3T3/A31 RIPA cell lysate, PC-12, Hep-G2, SW 620 or Jurkat RIPA cell lysates

Target description

125 kDa

Linkage

Replaces: 04-591

Physical form

Format: Purified
Protein G Purified
Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.1mM EDTA, 0.05% sodium azide before the addition of glycerol to 30%

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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    Y Shen et al.
    Molecular biology of the cell, 10(8), 2507-2518 (1999-08-06)
    The focal adhesion kinase (FAK) is discretely localized to focal adhesions via its C-terminal focal adhesion-targeting (FAT) sequence. FAK is regulated by integrin-dependent cell adhesion and can regulate tyrosine phosphorylation of downstream substrates, like paxillin. By the use of a
    B van de Water et al.
    The Journal of biological chemistry, 274(19), 13328-13337 (1999-05-01)
    The relationship between focal adhesion protein (FAK) activity and loss of cell-matrix contact during apoptosis is not entirely clear nor has the role of FAK in chemically induced apoptosis been studied. We investigated the status of FAK phosphorylation and cleavage
    Sanjeev K Baniwal et al.
    PloS one, 8(6), e62361-e62361 (2013-06-12)
    Prolactin-induced Protein (PIP), an aspartyl protease unessential for normal mammalian cell function, is required for the proliferation and invasion of some breast cancer (BCa) cell types. Because PIP expression is particularly high in the Luminal A BCa subtype, we investigated
    Nicoletta I Petridou et al.
    PloS one, 7(8), e42577-e42577 (2012-08-11)
    The Focal Adhesion Kinase is a well studied tyrosine kinase involved in a wide number of cellular processes including cell adhesion and migration. It has also been shown to play important roles during embryonic development and targeted disruption of the
    FAK/PYK2 promotes the Wnt/?-catenin pathway and intestinal tumorigenesis by phosphorylating GSK3?.
    Gao, C; Chen, G; Kuan, SF; Zhang, DH; Schlaepfer, DD; Hu, J
    eLife null
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