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MBD0017

Sigma-Aldrich

Inactivated Escherichia coli

Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml

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About This Item

UNSPSC Code:
41105500
NACRES:
NA.51

Quality Level

200

form

liquid

concentration

>10^8 bacteria/ml

technique(s)

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

suitability

suitable for restriction endonuclease digests, PCR amplification, Southern blots, and sequencing reactions

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses. Inactivated Escherichia coli standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.Escherichia coli is a gram negative, facultative aerobic, rod-shaped coliform bacterium. E. coli colonizes the infant gut within hours of birth and establishes itself as the most abundant facultative anaerobe of the human intestinal microflora for the remainder of life, equipped with the abilities to grow in the ever-changing environment in the gut and cope with the mammalian host interaction. Nevertheless, E. coli can survive in many different ecological habitats, including abiotic environments, and is considered a highly versatile species. Known habitats of E. coli include soil, water, sediment, and food. Some strains of E.coli have evolved and adapted to a pathogenic lifestyle and can cause different disease pathologies.

Read here how to use our standards to ensure data integrity for your microbiome research.

Application

Inactivated bacteria are provided at >10^8 bacteria/mL concentration in TE buffer pH 8.0. It is recommended to avoid freeze thaw cycles of this product.
Suitable for Quantitative standard for PCR, Sequencing and NGS

Features and Benefits

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

Physical form

Liquid -The inactivated bacteria is provided at >10^8 bacteria/mL in TE buffer pH 8.0

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Articles

DNA Standards for Metagenomic Research

DNA standards enhance metagenomics research integrity, offering precise species study and mixed community standards.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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