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Safety Information

F3040

Sigma-Aldrich

Monoclonal ANTI-FLAG® M1 antibody

clone M1, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-ddddk, Anti-dykddddk

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1 EA
CN¥6,110.23

CN¥6,110.23

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1 EA
CN¥6,110.23

About This Item

MDL number:
MFCD00212941
UNSPSC Code:
12352203
NACRES:
NA.32

CN¥6,110.23

Please contact Customer Service for Availability
Request a Bulk Order

biological source

(Bioreactor)

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M1, monoclonal

form

buffered aqueous solution

purified by

using Protein A

species reactivity

all

concentration

2-5 mg/mL

technique(s)

western blot: 10 μg/mL

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28476U28466U28467U
SLB®-5ms Capillary GC Column L × I.D. 20 m × 0.18 mm, df 0.18 μm

28564U

SLB®-5ms Capillary GC Column

SLB®-5ms Capillary GC Column L × I.D. 30 m × 0.25 mm, df 1.00 μm

28476U

SLB®-5ms Capillary GC Column

SLB®-5ms Capillary GC Column L × I.D. 15 m × 0.10 mm, df 0.10 μm

28466U

SLB®-5ms Capillary GC Column

SLB®-5ms Capillary GC Column L × I.D. 30 m × 0.25 mm, df 0.10 μm

28467U

SLB®-5ms Capillary GC Column

df

0.18 μm

df

1.00 μm

df

0.10 μm

df

0.10 μm

parameter

-60-340 °C temperature (isothermal), -60-360 °C temperature (programmed)

parameter

-60-340 °C temperature (isothermal), -60-360 °C temperature (programmed)

parameter

-60-340 °C temperature (isothermal), -60-360 °C temperature (programmed)

parameter

-60-340 °C temperature (isothermal), -60-360 °C temperature (programmed)

material

fused silica

material

fused silica

material

fused silica

material

fused silica

technique(s)

GC/MS: suitable, gas chromatography (GC): suitable (fast GC)

technique(s)

GC/MS: suitable, gas chromatography (GC): suitable

technique(s)

GC/MS: suitable, gas chromatography (GC): suitable (fast GC)

technique(s)

GC/MS: suitable, gas chromatography (GC): suitable

application(s)

agriculture
chemicals and industrial polymers
cleaning products
clinical
cosmetics
environmental
flavors and fragrances
food and beverages
forensics and toxicology
industrial hygiene
life science and biopharma
personal care
petroleum
pharmaceutical (small molecule)

application(s)

agriculture
chemicals and industrial polymers
cleaning products
clinical
cosmetics
environmental
flavors and fragrances
food and beverages
forensics and toxicology
industrial hygiene
life science and biopharma
personal care
petroleum
pharmaceutical (small molecule)

application(s)

agriculture
chemicals and industrial polymers
cleaning products
clinical
cosmetics
environmental
flavors and fragrances
food and beverages
forensics and toxicology
industrial hygiene
life science and biopharma
personal care
petroleum
pharmaceutical (small molecule)

application(s)

agriculture
chemicals and industrial polymers
cleaning products
clinical
cosmetics
environmental
flavors and fragrances
food and beverages
forensics and toxicology
industrial hygiene
life science and biopharma
personal care
petroleum
pharmaceutical (small molecule)

General description

The ANTI-FLAG M1 IgG2b mooclonal antibody binds to proteins with a FLAG marker at the free N-terminus.

Method of purification - Protein A

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

Monoclonal ANTI-FLAG® M1 antibody is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.


Chromatin immunoprecipitation (1 paper)


Immunofluorescence (1 paper)

Learn more product details in our FLAG® applications portal.

Biochem/physiol Actions

Binds to the FLAG epitope when it is located at the free amino-terminus of a fusion protein. Does not bind to Met-FLAG fusion proteins, so will not recognize unprocessed, cytoplasmically expressed proteins. Binding is Ca2+-dependent; the complex dissociates in the absence of calcium ions.

Physical form

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v).

Preparation Note

Dilute antibody to 10ug/mL in .05 M TBS, pH 7.4, containing 1 mM CaCl2.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品
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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000310613
    0000310613
    0000255099
    0000255085
    SLCR4808

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    Andrew Best et al.
    Nature communications, 5, 4760-4760 (2014-09-12)
    Alternative splicing--the production of multiple messenger RNA isoforms from a single gene--is regulated in part by RNA binding proteins. While the RBPs transformer2 alpha (Tra2α) and Tra2β have both been implicated in the regulation of alternative splicing, their relative contributions
    Mohammad Al Sorkhy et al.
    BMC cancer, 12, 45-45 (2012-01-28)
    Spy1 is a novel 'cyclin-like' activator of the G1/S transition capable of enhancing cell proliferation as well as inhibiting apoptosis. Spy1 protein levels are tightly regulated during normal mammary development and forced overexpression in mammary mouse models accelerates mammary tumorigenesis.
    Reem Berro et al.
    Journal of virology, 82(14), 7155-7166 (2008-05-16)
    The human immunodeficiency virus type 1 (HIV-1) Tat is a 14-kDa viral protein that acts as a potent transactivator by binding to the transactivation-responsive region, a structured RNA element located at the 5' end of all HIV-1 transcripts. Tat transactivates
    Chandrahasa R Yellaturu et al.
    The Journal of biological chemistry, 284(12), 7518-7532 (2009-01-23)
    The regulation of lipid homeostasis by insulin is mediated in part by the enhanced transcription of the gene encoding SREBP-1c (sterol regulatory element-binding protein-1c). Nascent SREBP-1c is synthesized and embedded in the endoplasmic reticulum (ER) and must be transported to
    Chandrahasa R Yellaturu et al.
    The Journal of biological chemistry, 284(46), 31726-31734 (2009-09-18)
    The regulation of lipid homeostasis by insulin is mediated in part by the enhanced transcription of the gene encoding sterol regulatory element-binding protein-1c (SREBP-1c). The nascent SREBP-1c is embedded in the endoplasmic reticulum (ER) and must be transported to the
    View All Related Papers

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