M8823
Anti-FLAG® M2 Magnetic Beads
affinity isolated antibody
Synonym(s):
Monoclonal ANTI-FLAG® M2, FLAG® magnetic affinity resin, FLAG® resin for high throughput, Flag® Affinity resin, Anti-ddddk, Anti-dykddddk
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About This Item
conjugate
magnetic beads
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
M2, monoclonal
form
suspension
shelf life
2 yr at -20 °C
analyte chemical class(es)
proteins
technique(s)
affinity chromatography: suitable
immunoprecipitation (IP): suitable
bead size
20-75 μm
matrix
superparamagnetic iron impregnated 4% agarose bead, with an average diameter of 50 μm.
isotype
IgG1
capacity
≥0.6 mg/mL binding capacity
shipped in
wet ice
storage temp.
−20°C
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General description
Application
Elution - FLAG® peptide, Glycine, pH 3.5, 3x FLAG® peptide
Learn more product details in our FLAG® application portal.
Features and Benefits
- Very rapid separation
- Significantly accelerated manipulations, such as repetitive washings
- Processing of multiple samples performed in plate formats
This leads to:
- Faster experimentation
- Better reproducibility
- More accurate quantitation of the proteins of interest
Physical form
Legal Information
Disclaimer
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Regulatory Information
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Articles
The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.
Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.
FLAG®表达系统是一种表达、纯化和检测重组融合蛋白的成熟方法。作为FLAG®的可靠供应商，Sigma®现提供FLAG标签抗体标记的免疫磁珠，用于免疫沉淀、蛋白纯化和蛋白互作研究。ANTI-FLAG® M2磁珠由ANTI-FLAG® M2鼠单克隆抗体与超顺磁铁包覆的4%琼脂糖微珠偶联而成，平均粒径50 µM。M2抗体可结合哺乳动物、细菌和植物提取物融合蛋白N端、Met-N端或C端的FLAG®肽。
比较anti-FLAG® M2磁珠的小规模FLAG®标签蛋白纯化的不同洗脱方法。
Related Content
默克分享基因组学、克隆学和大量分子生物学技术的发展使研究人员能在众多生物系统中表达异源蛋白。表达重组蛋白的能力为研究人员提供了广泛而强大的下游应用空间，以便进一步开展研究。
用于纯化重组蛋白的蛋白纯化技术、试剂和方法包括离子交换、排阻层析和蛋白亲和层析。
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