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P7582

Sigma-Aldrich

Amino-terminal FLAG-BAP™ Fusion Protein

Synonym(s):

Amino-terminal Fusion Protein, BAP Fusion Protein, FLAG-BAP Fusion Protein

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100 μG
¥3,773.82

¥3,773.82

Estimated to ship on2025年4月27日Details

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100 μG
¥3,773.82

About This Item

MDL number:
MFCD00130921
UNSPSC Code:
12352202
NACRES:
NA.32

¥3,773.82

Estimated to ship on2025年4月27日Details

form

liquid

Quality Level

200

mol wt

~49 kDa

shipped in

dry ice

storage temp.

−20°C

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This Item
P7457P5975F3290
氨基端 FLAG-BAP ™ 融合蛋白

P7582

氨基端 FLAG-BAP ™ 融合蛋白

Carboxy-terminal FLAG-BAP™ Fusion Protein

P7457

Carboxy-terminal FLAG-BAP™ Fusion Protein

Amino-terminal Met-FLAG-BAP™ Fusion Protein

P5975

Amino-terminal Met-FLAG-BAP™ Fusion Protein

FLAG® 肽 lyophilized powder

F3290

FLAG® 肽

mol wt

~49 kDa

mol wt

~49 kDa

mol wt

~49 kDa

mol wt

1012.97 Da

form

liquid

form

liquid

form

-

form

lyophilized powder

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Amino-terminal FLAG-BAP™ Fusion Protein is a 467 amino acid N-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular mass of 49.3kDa. The N-terminal FLAG-BAP Fusion Protein migrates as a 45−55kDa band by SDS-PAGE depending on electrophoresis conditions. FLAG-BAP™ Fusion Protein has biotechnological applications and is used in the screening of recombinant fusion proteins.[1]
The Amino-terminal FLAG-BAP Fusion Protein is a control prorein used to confirm the funtional integrity of the anti-FLAG M1 and M2 monoclonal antibodies in applications such as western blotting, immunoprecipitation, ELISA, electron microscopy, and FACS.

Application

Amino-terminal FLAG-BAP™ Fusion Protein has been used as a standard in enzyme-linked immunosorbent assay (ELISA) for osteopontin quantification[2] and in western blotting based quantification of Flag-Wnt8[3] and Drosophila melanogaster transcription factor interactome.[4]
Learn more product details in our FLAG® application portal.

Other Notes

Control protein

Physical form

Protein is supplied in 10 mM Tris, 120 mM NaCl,
0.05 mM ZnCl2 in 50% glycerol, pH 8.0.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCK2898
    SLCH4561
    SLCG2086
    SLBW2067
    SLBZ4309

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    Find documentation for the products that you have recently purchased in the Document Library.

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    A Comprehensive Drosophila melanogaster Transcription Factor Interactome
    Shokri L, et al.
    Cell Reports, 27(3), 955-970 (2019)
    BAP-fusion: A versatile molecular probe for biotechnology research
    Biotechnology: Research, Technology and Applications, 327-345 (2008)
    Exploring the separation power of mixed-modal resins for purification of recombinant osteopontin from clarified Escherichia coli lysates
    Guo S, et al.
    Biotechnology Progress, 35(1), e2722-e2722 (2019)
    Noggin4 is a long-range inhibitor of Wnt8 signalling that regulates head development in Xenopus laevis
    Eroshkin FM, et al.
    Scientific Reports, 6, 23049-23049 (2016)
    Daniela Castiglia et al.
    Biotechnology for biofuels, 9, 154-154 (2016-07-28)
    Biofuels production from plant biomasses is a complex multi-step process with important economic burdens. Several biotechnological approaches have been pursued to reduce biofuels production costs. The aim of the present study was to explore the production in tobacco plastome of
    View All Related Papers

    Articles

    Anti-FLAG® M2 Magnetic Beads

    Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

    Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

    Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

    Protocols

    Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

    Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

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    Protein Purification

    Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

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    Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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