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Safety Information

B3111

Sigma-Aldrich

ANTI-FLAG® M2 antibody, Mouse monoclonal

Clone M2, purified from hybridoma cell culture in bioreactor

Synonym(s):

Anti-ddddk, Anti-dykddddk, M2 clone ANTI-FLAG

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250 REACTIONS
CN¥2,108.32
1250 REACTIONS
CN¥9,461.07

CN¥2,108.32

Please contact Customer Service for Availability
New, lower price on this item!
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250 REACTIONS
CN¥2,108.32
1250 REACTIONS
CN¥9,461.07

About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

CN¥2,108.32

Please contact Customer Service for Availability
New, lower price on this item!

biological source

mouse

antibody form

purified immunoglobulin (purified IgG1 subclass)

clone

M2, monoclonal

shelf life

4 yr

purified by

using Protein A

storage temp.

−20°C

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form

liquid

form

liquid

form

liquid

form

liquid

usage

sufficient for 1250 reactions

usage

sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions

usage

sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions

usage

sufficient for 500 reactions

feature

dNTPs included

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

storage condition

protect from light

storage condition

protect from light

storage condition

protect from light

storage condition

protect from light

color

light blue

color

light blue

color

light blue

color

light blue

General description

Monoclonal ANTI-FLAG M2 is a purified immunoglobulin, IgG1, monoclonal antibody, purified from culture supernatant of hybridoma cells, that binds to FLAG® fusion proteins. Unlike ANTI-FLAG M1 antibody, the M2 antibody will recognize the FLAG sequence at the N-terminus, Met-N-terminus, C-terminus, or at an internal site of FLAG fusion proteins. Monoclonal ANTI-FLAG M2 is useful for identification and capture of FLAG fusion proteins by common immunological procedures such as Western blots and immunoprecipitation. It is also useful for affinity purification of FLAG fusion proteins when bound to a solid support.

form: solution pH 7.4, containing 15 mM sodium azide
concentration: 3.0-5.0 mg/mL

Application

IB, IF, IP, FACS, ELISA
Antibody is recommended for use in several applications such as immunoblotting, immunoprecipitation, immunofluorescence, flow cytometry, and ELISA.

Learn more product details in our FLAG® application portal.

Packaging

polypropylene screw cap vial

Preparation Note

Dilute the antibody solution from 0.5-10 ug/mL in specified buffer

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    Lot/Batch Number
    0000420002
    0000420002
    0000395436
    0000388873
    0000346721

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