B3111
ANTI-FLAG® M2 antibody, Mouse monoclonal
Clone M2, purified from hybridoma cell culture in bioreactor
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Synonym(s):
Anti-ddddk, Anti-dykddddk, M2 clone ANTI-FLAG
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
mouse
antibody form
purified immunoglobulin (purified IgG1 subclass)
clone
M2, monoclonal
shelf life
4 yr
purified by
using Protein A
storage temp.
−20°C
Related Categories
General description
Monoclonal ANTI-FLAG M2 is a purified immunoglobulin, IgG1, monoclonal antibody, purified from culture supernatant of hybridoma cells, that binds to FLAG® fusion proteins. Unlike ANTI-FLAG M1 antibody, the M2 antibody will recognize the FLAG sequence at the N-terminus, Met-N-terminus, C-terminus, or at an internal site of FLAG fusion proteins. Monoclonal ANTI-FLAG M2 is useful for identification and capture of FLAG fusion proteins by common immunological procedures such as Western blots and immunoprecipitation. It is also useful for affinity purification of FLAG fusion proteins when bound to a solid support.
form: solution pH 7.4, containing 15 mM sodium azide
concentration: 3.0-5.0 mg/mL
form: solution pH 7.4, containing 15 mM sodium azide
concentration: 3.0-5.0 mg/mL
Application
IB, IF, IP, FACS, ELISA
Antibody is recommended for use in several applications such as immunoblotting, immunoprecipitation, immunofluorescence, flow cytometry, and ELISA.
Learn more product details in our FLAG® application portal.
Antibody is recommended for use in several applications such as immunoblotting, immunoprecipitation, immunofluorescence, flow cytometry, and ELISA.
Learn more product details in our FLAG® application portal.
Packaging
polypropylene screw cap vial
Preparation Note
Dilute the antibody solution from 0.5-10 ug/mL in specified buffer
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Runming Zeng et al.
Experimental biology and medicine (Maywood, N.J.), 246(6), 644-653 (2020-12-11)
Osteoarthritis (OA), the most prevalent form of arthritis disease, is characterized by destruction of articular cartilage, osteophyte development, and sclerosis of subchondral bone. Transcription factors Janus kinase 1/signal transducer and activator of transcription 3 (JAK1/STAT3) and Forkhead box M1 (FOXM1)
Yi-Min Chu et al.
Frontiers in oncology, 12, 900166-900166 (2022-10-04)
DLC1 (deleted in liver cancer-1) is downregulated or deleted in colorectal cancer (CRC) tissues and functions as a potent tumor suppressor, but the underlying molecular mechanism remains elusive. We found that the conditioned medium (CM) collected from DLC1-overexpressed SW1116 cells
Xin Wu et al.
mBio, 13(2), e0040222-e0040222 (2022-03-29)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection triggers cytokine-mediated inflammation, leading to a myriad of clinical presentations in COVID-19. The SARS-CoV-2 open reading frame 8 (ORF8) is a secreted and rapidly evolving glycoprotein. Patients infected with SARS-CoV-2 variants with
Weixiang Bian et al.
STAR protocols, 3(3), 101569-101569 (2022-07-26)
Identification of protein interactors is fundamental to understanding their functions. Here, we describe a modified protocol for tandem affinity purification coupled with mass spectrometry (TAP/MS), which includes two-step purification. We detail the S-, 2×FLAG-, and Streptavidin-Binding Peptide (SBP)- tandem tags
Junmei Cairns et al.
NAR cancer, 2(4), zcaa039-zcaa039 (2021-01-02)
Cell division cycle 25 (CDC25) dual specificity phosphatases positively regulate the cell cycle by activating cyclin-dependent kinase/cyclin complexes. Here, we demonstrate that in addition to its role in cell cycle regulation, CDC25B functions as a regulator of protein phosphatase 2A
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